| Full text | |
| Author(s): Show less - |
Ibanez-Redin, Gisela
[1]
;
Furuta, Roberto H. M.
[1]
;
Wilson, Deivy
[1]
;
Shimizu, Flavio M.
[1, 2]
;
Materon, Elsa M.
[1, 3]
;
Rebolho Batista Arantes, Lidia Maria
[4]
;
Melendez, Matias E.
[4]
;
Carvalho, Andre L.
[4]
;
Reis, Rui Manuel
[4, 5, 6]
;
Chaur, Manuel N.
[7]
;
Goncalves, Debora
[1]
;
Oliveira, Jr., Osvaldo N.
[1]
Total Authors: 12
|
| Affiliation: | [1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Chem, BR-13565970 Sao Carlos, SP - Brazil
[4] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos, SP - Brazil
[5] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal
[6] Univ Minho, Hlth Sci Sch, Life & Hlth Sci Res Inst ICVS, Braga - Portugal
[7] Univ Valle, Dept Quim, Cl 13 100-00, Cali - Colombia
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Materials Science & Engineering C-Materials for Biological Applications; v. 99, p. 1502-1508, JUN 2019. |
| Web of Science Citations: | 4 |
| Abstract | |
Nanostructured capacitive biosensors, combined with inexpensive fabrication technologies, may provide simple, sensitive devices for detecting clinically relevant cancer biomarkers. Herein, we report a novel platform for detecting the pancreatic cancer biomarker CA19-9 using low-cost screen-printed interdigitated electrodes (SPIDEs). The SPIDEs were modified by carbon nano-onions (CNOs) and graphene oxide (GO) films, on which a layer of anti-CA19-9 antibodies was immobilized. The modification with CNOs and GO significantly improved the analytical performance of the biosensor, which displayed superior results to those prepared only with GO. The biossensor exhibited high reproducibility and a relatively low limit of detection of 0.12 U mL(-1). Using these devices in combination with information visualization methods we were able to detect CA19-9 in whole cell lysates of colorectal adenocarcinoma. The fabrication of these low-cost, disposable inummosensors is a successful attempt to explore CNOs in capacitive biosensors, which may be extended for detection of different cancer biomarkers. (AU) | |
| FAPESP's process: | 17/12096-3 - Production of bounded interdigitated electrodes for biosensors for detection of cancer biomarkers |
| Grantee: | Roberto Hiroshi Matos Furuta |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 13/14262-7 - Nanostructured films from biologically-relevant materials |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/01770-0 - Development of analytical tools based on electronic tongues for simultaneous detection of bacterial threats |
| Grantee: | Deivy Wilson Masso |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/00991-5 - Study of Detoxification Enzymes Interaction with chemotherapeutic drugs and its relationship with chemoresistance |
| Grantee: | Elsa María Materón Vásques |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/15543-7 - Biosensors to detect Escherichia coli using the concept of an extended electronic tongue |
| Grantee: | FLAVIO MAKOTO SHIMIZU |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |