| Full text | |
| Author(s): Show less - |
Moraes, Ariana de Souza
[1, 2, 3]
;
Brum, Doralina Guimaraes
[3, 4]
;
Magalhaes Ierich, Jessica Cristiane
[1, 2, 3]
;
Higa, Akemi Martins
[1, 2, 3]
;
Jabur Assis, Amanda Stefanie
[1, 3]
;
Miyazaki, Celina Massumi
[1]
;
Shimizu, Flavio Makoto
[5]
;
Peroni, Luis Antonio
[6]
;
Machini, M. Teresa
[7]
;
Barreira, Amilton Antunes
[8]
;
Ferreira, Marystela
[1]
;
Oliveira Jr, Osvaldo N.
;
Leite, Fabio Lima
[1, 3]
Total Authors: 13
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Phys Chem & Math, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Carlos, Nanoneurobiophys Res Grp GNN, BR-18052780 Sorocaba, SP - Brazil
[4] Sao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP - Brazil
[5] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
[6] Rheabiotech Lab Res & Dev, Campinas, SP - Brazil
[7] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil
[8] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav Sci, Ribeirao Preto, SP - Brazil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 9, NOV 6 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4(61-70), AQP4(131-140), AQP4(141-150), and AQP4(201-210)) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4(61-70)-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4(61-70) sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4(61-70)-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target. (AU) | |
| FAPESP's process: | 16/19387-0 - LbL (LAYER-BY-LAYER) and Langmuir-Blodgett (LB) nanostructured films with emodin for systems carriers. |
| Grantee: | Marystela Ferreira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/50869-6 - INCT 2014: on Organic Electronics |
| Grantee: | Roberto Mendonça Faria |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/14262-7 - Nanostructured films from biologically-relevant materials |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing |
| Grantee: | Celina Massumi Miyazaki |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/50839-4 - Development of new method for detection of anti-aquaporin-4 for the study of neuromyelitis optica |
| Grantee: | Doralina Guimarães Brum Souza |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/12082-4 - Molecular modeling of the antigen-antibody complex to the investigations of autoimmune demyelinating diseases |
| Grantee: | Jéssica Cristiane Magalhães Ierich |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/05283-6 - Nanoimmunosensors development to neuromyelitis optica survey and detection |
| Grantee: | Ariana de Souza Moraes dos Santos |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 15/06847-0 - Synthesis and characterization of silver nanoparticles applied to immunoglobulins G detection |
| Grantee: | Akemi Martins Higa |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 15/14360-4 - Magnetic bionanocatalysts: preparation, characterization, properties and use in protein and peptide chemistry |
| Grantee: | Maria Teresa Machini |
| Support Opportunities: | Regular Research Grants |