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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders

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Moraes, Ariana de Souza [1, 2, 3] ; Brum, Doralina Guimaraes [3, 4] ; Magalhaes Ierich, Jessica Cristiane [1, 2, 3] ; Higa, Akemi Martins [1, 2, 3] ; Jabur Assis, Amanda Stefanie [1, 3] ; Miyazaki, Celina Massumi [1] ; Shimizu, Flavio Makoto [5] ; Peroni, Luis Antonio [6] ; Machini, M. Teresa [7] ; Barreira, Amilton Antunes [8] ; Ferreira, Marystela [1] ; Oliveira Jr, Osvaldo N. ; Leite, Fabio Lima [1, 3]
Total Authors: 13
[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Carlos, Nanoneurobiophys Res Grp GNN, BR-18052780 Sorocaba, SP - Brazil
[4] Sao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP - Brazil
[5] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
[6] Rheabiotech Lab Res & Dev, Campinas, SP - Brazil
[7] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil
[8] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav Sci, Ribeirao Preto, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, NOV 6 2019.
Web of Science Citations: 0

A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4(61-70), AQP4(131-140), AQP4(141-150), and AQP4(201-210)) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4(61-70)-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4(61-70) sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4(61-70)-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target. (AU)

FAPESP's process: 16/19387-0 - LbL (LAYER-BY-LAYER) and Langmuir-Blodgett (LB) nanostructured films with emodin for systems carriers.
Grantee:Marystela Ferreira
Support type: Regular Research Grants
FAPESP's process: 14/50869-6 - INCT 2014: on Organic Electronics
Grantee:Roberto Mendonça Faria
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing
Grantee:Celina Massumi Miyazaki
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/50839-4 - Development of new method for detection of anti-aquaporin-4 for the study of neuromyelitis optica
Grantee:Doralina Guimarães Brum Souza
Support type: Regular Research Grants
FAPESP's process: 14/12082-4 - Molecular modeling of the antigen-antibody complex to the investigations of autoimmune demyelinating diseases
Grantee:Jéssica Cristiane Magalhães Ierich
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/05283-6 - Nanoimmunosensors development to neuromyelitis optica survey and detection
Grantee:Ariana de Souza Moraes
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/06847-0 - Synthesis and characterization of silver nanoparticles applied to immunoglobulins G detection
Grantee:Akemi Martins Higa
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/14360-4 - Magnetic bionanocatalysts: preparation, characterization, properties and use in protein and peptide chemistry
Grantee:Maria Teresa Machini
Support type: Regular Research Grants