Food safety evaluation for the use of albendazole in fish: residual depletion profile and withdrawal period estimation

Author(s)

Vellosa Portela, Ana Carolina ^[1] ; Fernandes Silveira, Juliana Grell ^[1] ; Damaceno, Marina Alves ^[1] ; Baldo da Silva, Agnaldo Fernando ^[1] ; de Jesus, Raphael Barbetta ^[2] ; Pilarski, Fabiana ^[2] ; Gadaj, Anna ^[3] ; Mooney, Mark H. ^[3] ; Rizzato Paschoal, Jonas Augusto ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Paulista Unesp, Ctr Aquicultura Unesp, Jaboticabal - Brazil

^[3] Queens Univ Belfast, Inst Global Food Secur, Sch Biol Sci, Belfast, Antrim - North Ireland

• Document type: Journal article
• Source: Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure; v. 37, n. 4 FEB 2020.
• Web of Science Citations: 0

Abstract

Due to the lack of drugs regulated for aquaculture, we have evaluated the use of albendazole (ABZ) - a potential drug to be regulated for fish - under food safety perspectives assessing the depletion profile of ABZ and its main metabolites (albendazole sulphoxide - ABZSO, albendazole sulphone - ABZSO(2) and albendazole amino sulphone - ABZ-2-NH2SO2) in fish fillets (muscle and skin) after single dose oral administration of 10 mg ABZ kg(-1) body weight. For the drug administration, a suitable procedure for ABZ incorporation into fish feed was employed, obtaining good homogeneity of ABZ concentration among feed pellets (CV<4.1%) and low drug leaching when medicated feed remained in the water for up to 60 min (<2.7%). After medication, fish were euthanised at 8, 12, 24, 48, 72, 96 and 120 h and fillets collected. Depletion studies in various fish species (patinga and tilapia) were conducted simultaneously, under water temperature at 30.4 +/- 0.3 degrees C and pH 6.8 +/- 0.1. The highest concentrations for the sum of residues (ABZ, ABZSO, ABZSO(2) and ABZ-2-NH2SO2) in fish fillet were 1210 ng g(-1) in patinga and 637 ng g(-1) in tilapia. Under the employed rearing conditions, the obtained results did not indicate a requirement for a minimum withdrawal period to be proposed for tilapia considering the maximum residue limit of 100 mu g g(-1), since the determined residual concentration was <LOQ (10 ng g(-1)) from 24 h post medication treatment. For patinga, the estimated minimum withdrawal period was 3 days. With the well-recognised demands for new alternative veterinary drugs for aquaculture use by fish farmers, this study offers important evidence for consideration of ABZ use in tilapia and patinga taking into account food safety issues. (AU)

• FAPESP's process: 12/18334-0 - Veterinary drug residues in food: multiclass analytical methods employing LC-MS/MS, exposition data and residual depletion study
• Grantee: Jonas Augusto Rizzato Paschoal
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/16593-9 - Estimation and evaluation of withdrawal periods for albendazole and its metabolites in fish
• Grantee: Ana Carolina Vellosa Portela
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/50755-0 - Metaqua: metabolite/metabolomic profiling in aquaculture research with a view to maintaining food safety / security and animal / human health
• Grantee: Jonas Augusto Rizzato Paschoal
• Support Opportunities: Regular Research Grants