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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanoimmunosensor based on atomic force spectroscopy to detect anti-myelin basic protein related to early-stage multiple sclerosis

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Garcia, Pamela Soto [1, 2] ; Brum, Doralina Guimaraes [3] ; Oliveira Jr, Osvaldo N. ; Higa, Akemi Martins [1, 2] ; Magalhaes Ierich, Jessica Cristiane [1, 2] ; Moraes, Ariana de Souza [1, 2] ; Shimizu, Flavio Makoto [4] ; Okuda-Shinagawa, Nancy M. [5] ; Peroni, Luis Antonio [6] ; da Gama, Paulo Diniz [7] ; Teresa Machini, M. [5] ; Leite, Fabio Lima [1]
Total Authors: 12
[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, Nanoneurobiophys Res Grp, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo, SP - Brazil
[3] Sao Paulo State Univ, Dept Neurol Psychol & Psychiat, BR-18618687 Botucatu, SP - Brazil
[4] Oliveira Jr, Jr., Osvaldo N., Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
[5] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP - Brazil
[6] Rheabiotech Lab Res & Dev, BR-13084791 Campinas, SP - Brazil
[7] Pontifical Catholic Univ Sao Paulo, BR-18030010 Sorocaba, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: ULTRAMICROSCOPY; v. 211, APR 2020.
Web of Science Citations: 0

Multiple Sclerosis (MS) is a chronic inflammatory disorder in the central nervous system for which biomarkers for diagnosis still remain unknown. One potential biomarker is the myelin basic protein. Here, a nanoimmunosensor based on atomic force spectroscopy (AFS) successfully detected autoantibodies against the MBP85-99 peptide from myelin basic protein. The nanoimmunosensor consisted of an atomic force microscope tip functionalization with MBP85-99 peptide, which was made to interact with a mica surface coated either with a layer of anti-MBP85-99 (positive control) or samples of cerebrospinal fluid (CSF) from five multiple sclerosis (MS) patients at different stages of the disease and five non-MS subjects. The adhesion forces obtained from AFS pointed to a high concentration of anti-MBP85-99 for the two patients at early stages of relapsing-remitting multiple sclerosis (RRMS), which were indistinguishable from the positive control. In contrast, considerably lower adhesion forces were measured for all the other eight subjects, including three MS patients with longer history of the disease and under treatment, without episodes of acute MS activity. We have also shown that the average adhesion force between MBP85-99 and anti-MBP85-99 is compatible with the value estimated using steered molecular dynamics. Though further tests will be required with a larger cohort of patients, the present results indicate that the nanoimmunosensor may be a simple tool to detect early-stage MS patients and be useful to understand the molecular mechanisms behind MS. (AU)

FAPESP's process: 14/50869-6 - INCT 2014: on Organic Electronics
Grantee:Roberto Mendonça Faria
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/50839-4 - Development of new method for detection of anti-aquaporin-4 for the study of neuromyelitis optica
Grantee:Doralina Guimarães Brum Souza
Support type: Regular Research Grants
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/12082-4 - Molecular modeling of the antigen-antibody complex to the investigations of autoimmune demyelinating diseases
Grantee:Jéssica Cristiane Magalhães Ierich
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/21530-0 - Imnunonanosensors based on atomic force microscopy for multiple sclerosis studies
Grantee:Pâmela Soto Garcia
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/06847-0 - Synthesis and characterization of silver nanoparticles applied to immunoglobulins G detection
Grantee:Akemi Martins Higa
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/14360-4 - Magnetic bionanocatalysts: preparation, characterization, properties and use in protein and peptide chemistry
Grantee:Maria Teresa Machini
Support type: Regular Research Grants