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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simultaneous extraction and separation of bioactive compounds from apple pomace using pressurized liquids coupled on-line with solid-phase extraction

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da Silva, Laise C. [1] ; Souza, Mariana C. [1] ; Sumere, Beatriz R. [1] ; Silva, Luiz G. S. [1] ; da Cunha, Diogo T. [1] ; Barbero, Gerardo F. [2] ; Bezerra, Rosangela M. N. [1] ; Rostagno, Mauricio A. [1]
Total Authors: 8
[1] Univ Estadual Campinas, Sch Appl Sci FCA, Multidisciplinary Lab Food & Hlth LabMAS, UNICAMP, Rua Pedro Zaccaria 1300, BR-13484350 Limeira, SP - Brazil
[2] Univ Cadiz, Fac Sci, Dept Analyt Chem, IVAGRO, Agrifood Campus Int Excellence CeiA3, POB 40, Cadiz 11510 - Spain
Total Affiliations: 2
Document type: Journal article
Source: Food Chemistry; v. 318, JUL 15 2020.
Web of Science Citations: 0

The objective of this work was the development of an on-line extraction/fractionation method based on the coupling of pressurized liquid extraction and solid-phase extraction for the separation of phenolic compounds from apple pomace. Several variables of the process were evaluated, including the amount of water of the first stage (0-120 mL), temperature (60-80 degrees C), solid-phase extraction adsorbent (Sepra, Isolute, Strata X and Oasis) and activation/elution solvent (methanol and ethanol). The best results were observed with the adsorbent Sepra. The temperature had a small effect on recovery, but significant differences were observed for phlorizin and a quercetin derivative. Results indicate that ethanol can be used to replace methanol as an activation, extraction/elution solvent. While using mostly green solvents (water, ethanol, and a small amount of methanol that could be reused), the developed method produced higher or similar yields of acids (2.85 +/- 0.19 mg/g) and flavonoids (0.97 +/- 0.11 mg/g) than conventional methods. (AU)

FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
Grantee:Maurício Ariel Rostagno
Support type: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 16/19930-6 - Evaluation of the extraction with pressurized liquid assisted by ultrasound coupled to the solid phase extraction for the purification of phenolic compounds from tea (Camellia sinensis)
Grantee:Mariana Corrêa de Souza
Support type: Scholarships in Brazil - Master
FAPESP's process: 18/17089-8 - Separation of bioactive compounds from black tea and mate by on-line coupling of pressurized liquid extraction with an adsorbent and evaluation of their antimicrobial potential
Grantee:Mariana Corrêa de Souza
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/24537-0 - Phytochemical evaluation of apple (Malus domestica) industrial residue from extraction and separation by a two-dimensional system
Grantee:Laise Capelasso da Silva
Support type: Scholarships in Brazil - Doctorate (Direct)