Characterization of pomegranate peel extracts obtained using different solvents and their effects on cell cycle and apoptosis in leukemia cells

Author(s)

Tamborlin, Leticia ^{[1, 2]} ; Sumere, Beatriz Rocchetti ^[3] ; de Souza, Mariana Correa ^[3] ; Pestana, Nathalie Fortes ^[3] ; Aguiar, Ana Carolina ^[4] ; Eberlin, Marcos Nogueira ^{[4, 5]} ; Simabuco, Fernando Moreira ^[3] ; Rostagno, Mauricio Ariel ^[3] ; Luchessi, Augusto Ducati ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Lab Biotechnol BioTech, Sch Appl Sci FCA, UNICAMP, Limeira - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci IB, Rio Claro - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Multidisciplinary Lab Food & Hlth LabMAS, Sch Appl Sci FCA, Limeira - Brazil

^[4] Univ Estadual Campinas, Inst Chem IQ, ThoMSon Mass Spectrometry Lab, UNICAMP, Campinas - Brazil

^[5] Univ Prebiteriana Mackenzie, Sch Engn PPGEMN, MackMass Lab, Sao Paulo - Brazil

• Document type: Journal article
• Source: FOOD SCIENCE & NUTRITION; v. 8, n. 10 AUG 2020.
• Web of Science Citations: 0

Abstract

Pomegranate (Punica granatum L.) has been used in traditional herbal medicine by several cultures as an anti-inflammatory, antioxidant, antihyperglycemic, and for treatment and prevention of cancer and other diseases. Different parts of the fruit, extraction methods, and solvents can define the chemical profile of the obtained extracts and their biological activities. This study aimed to characterize the chemical profile of peel extracts collected using different extraction solvents and their biological effects on the cell cycle and apoptosis of THP-1 leukemic cells. Aqueous extract presented the highest content of punicalagins (alpha pun = 562.26 +/- 47.14 mg/L and beta pun = 1,251.13 +/- 22.21 mg/L) and the lowest content of ellagic acid (66.38 +/- 0.21 mg/L), and it promoted a significant impairment of the cell cycle S phase. In fact, punicalagin-enriched fraction, but not an ellagic acid-enriched fraction, caused an S phase cell cycle arrest. All extracts increased the number of apoptotic cells. Punicalagin-enriched fraction increased the percentage of cells with fragmented DNA, which was intensified by ellagic acid combination. The treatment combining punicalagin and ellagic acid fractions increased the apoptotic cleaved PARP1 protein and reduced the activation of the growth-related mTOR pathway. Thus, these results evidence that solvent choice is critical for the phenolic compounds profile of pomegranate peel extracts and their biological activities. (AU)

• FAPESP's process: 18/17089-8 - Separation of bioactive compounds from black tea and mate by on-line coupling of pressurized liquid extraction with an adsorbent and evaluation of their antimicrobial potential
• Grantee: Mariana Corrêa de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/18119-0 - Ultrasound emulsification of biquinho pepper (Capsicum chinense) extracts obtained by supercritical fluid extraction as an alternative to increase its bioactivity
• Grantee: Ana Carolina de Aguiar
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/06951-3 - Screening of the amino acid hypusine molecular actions
• Grantee: Augusto Ducati Luchessi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/18095-0 - Study of the eukaryotic translation initiation factor 5A (eIF5A) in mammals
• Grantee: Augusto Ducati Luchessi
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/14818-9 - Study of molecular targets important for the control of cancer metabolism: the mTOR/S6K pathway as a central role
• Grantee: Fernando Moreira Simabuco
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 17/21914-1 - Functional characterization of the amino acid hypusine
• Grantee: Leticia Tamborlin
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants