Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications

• Grant number: 18/15549-1
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• Start date: October 01, 2019
• End date: March 31, 2026
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Giuseppe Palmisano
• Grantee: Giuseppe Palmisano
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Cláudio Romero Farias Marinho ; Maria Julia Manso Alves
• Associated research grant: 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications, AP.JP
• Associated research grant(s): 25/13818-9 - Developing a respiratory diagnostic test using exhaled breath condensate proteins: an accessible and non-invasive approach., AP.R; 24/02146-7 - Discovery and validation of de novo generated endogenous biomarkers for the diagnosis of Chagas disease, AP.R; 20/04923-0 - SARS-CoV-2 glycosylation: a blueprint structural insight for understanding COVID-19 pathogenesis, AP.R; 19/08796-5 - Novel nanoemulsion for enhanced efficacy and better safety of benznidazole in the treatment of Chagas Disease, AP.R SPRINT
• Associated scholarship(s): 25/00049-7 - Identification of protein glycosylation-dependent alterations in the serum of Type 2 diabetic individuals with cognitive decline using an analytical, computational and biochemical approach, BP.PD; 24/03969-7 - Development of analytical techniques in proteomics and glycoproteomics applied to Trypanosoma cruzi study., BP.TT; 23/04937-9 - Technical development in proteomics: strategies and analytical techniques, BP.TT; + associated scholarships 22/09915-0 - Modulation of host N-glycosylation machinery during Trypanosoma cruzi infection, BP.DR; 22/00796-9 - Unravelling the biological role of T. cruzi protein glycosylation, BP.PD; 21/14179-9 - The role of the endoplasmic reticulum in viral and parasitic virulence, BP.DD; 21/14751-4 - Novel mass spectrometry-based technologies for post-translational modifications analysis, BP.PD; 21/00140-3 - Understanding the role of protein arginylation in health and disease through analytical and biological approaches, BP.DR - associated scholarships

Abstract

Chagas Disease, caused by the protozoan parasite T. cruzi, is a neglected disease, which can lead to Cardiomyopathy, neurological complications and megaviscera. Over 100 years after its description, this disease affects 8 million people and most of them do not know that they are infected. Although endemic in the South America, it has been recognized a worldwide public health problem due to global population migration. Despite the great efforts to control T. cruzi transmission and introduce novel therapeutic options, one of the unmet needs is the development of novel sensitive and specific diagnostic markers for treatment response and disease progression. Moreover, a better understanding of the T. cruzi-host interaction will aid in prioritize novel therapeutic targets. This project is divided in three parts: 1) analytical method development for post-translational modifications (PTMs); 2) elucidation of the role of protein glycosylation and arginylation in the T. cruzi-host interaction and 3) biomarker discovery for Chagas Disease. The development of novel analytical methods for PTMs analysis will offer the possibility to detect protein modifications in complex biological systems with high accuracy and sensitivity. In particular, the identification of protein glycosylation of different T. cruzi strains belonging to the seven DTUs will be evaluated and associated to the expression of specific glycosyltransferases. The large-scale analysis of the cell surface glycoproteome and glycome combined with functional assays will be used as a molecular determinant to link the T. cruzi glycosylation enzymes expression and phenotype. The characterization of protein arginylation in T. cruzi and the Tc-Ate1 enzyme involved in this process will allow us to understand the role of this poorly explored PTM in Chagas Disease biology. The third part of the project will be focused on implementing a diagnostic platform for urinary protein biomarker discovery in Chagas Disease. (AU)

Scientific publications

(62)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

CHATTERJEE, SAYANTANI; LEE, LING Y.; KAWAHARA, REBECA; ABRAHAMS, JODIE L.; ADAMCZYK, BARBARA; ANUGRAHAM, MERRINA; ASHWOOD, CHRISTOPHER; SUMER-BAYRAKTAR, ZEYNEP; BRIGGS, MATTHEW T.; CHIK, JENNY H. L.; et al. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. PROTEOMICS, v. 19, n. 21-22, SI, NOV 2019. (17/03010-8, 18/15549-1, 15/02866-0, 14/06863-3)

CAPELLI-PEIXOTO, JANAINA; MULE, SIMON NGAO; TANO, FABIA TOMIE; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteomics and Leishmaniasis: Potential Clinical Applications. PROTEOMICS CLINICAL APPLICATIONS, v. 13, n. 6, AUG 2019. (17/26197-6, 17/04287-3, 14/26777-4, 14/06863-3, 18/18257-1, 18/15549-1, 17/04032-5)

MARTINS, LAYLA FARAGE; DOS SANTOS JUNIOR, ARIOSVALDO PEREIRA; NICASTRO, GIANLUCCA GONCALVES; SCHEUNEMANN, GABY; ANGELI, CLAUDIA BLANES; ROSSI, FERNANDO PACHECO NOBRE; QUAGGIO, RONALDO BENTO; PALMISANO, GIUSEPPE; SGRO, GERMAN GUSTAVO; ISHIDA, KELLY; et al. Phages ZC01 and ZC03 require type-IV pilus for Pseudomonas aeruginosa infection and have a potential for therapeutic applications. MICROBIOLOGY SPECTRUM, v. N/A, p. 17-pg., 2024-10-29. (21/10577-0, 20/04923-0, 18/18257-1, 18/15549-1, 22/11334-6)

COSTA, GABRIEL CERQUEIRA ALVES; TORQUATO, RICARDO JOSE SOARES; GOMES, VINICIUS DE MORAIS; ROSA-FERNANDES, LIVIA; PALMISANO, GIUSEPPE; TANAKA, APARECIDA SADAE. Functional characterization of a cystatin A from the bat Myotis davidii. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 274, p. 6-pg., 2024-06-29. (20/05451-4, 20/04923-0, 18/18257-1, 19/03779-5, 18/15549-1, 20/02433-5)

LIMA, GUILHERME MEIRA; EFFER, BRIAN; BIASOTO, HENRIQUE PELLIN; FEIJOLI, VERONICA; PESSOA, ADALBERTO; PALMISANO, GIUSEPPE; MONTEIRO, GISELE. Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis. Biochemical Engineering Journal, v. 156, p. 11-pg., 2020-04-15. (14/06863-3, 16/15787-4, 18/15549-1, 18/18257-1, 15/07749-2, 13/08617-7)

LAZARI, LUCAS CARDOSO; GHILARDI, FABIO DE ROSE; ROSA-FERNANDES, LIVIA; ASSIS, DIEGO M.; NICOLAU, JOSE CARLOS; SANTIAGO, VERONICA FEIJOLI; DALCOQUIO, TALIA FALCAO; ANGELI, CLAUDIA B.; BERTOLIN, ADRIADNE JUSTI; MARINHO, CLAUDIO R. F.; et al. Prognostic accuracy of MALDI-TOF mass spectrometric analysis of plasma in COVID-19. LIFE SCIENCE ALLIANCE, v. 4, n. 8, AUG 2021. (20/04705-2, 18/15549-1, 17/03966-4, 15/26722-8, 18/20468-0, 20/04923-0, 18/18257-1)

COUTINHO, JOAO V. P.; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; DE OLIVEIRA, GILBERTO SANTOS; MANCHOLA, NUBIA CAROLINA; SANTIAGO, VERONICA FEIJOLI; COLLI, WALTER; WRENGER, CARSTEN; MANSO ALVES, MARIA JULIA; PALMISANO, GIUSEPPE. The thermal proteome stability profile of Trypanosoma cruzi in epimastigote and trypomastigote life stages. JOURNAL OF PROTEOMICS, v. 248, SEP 30 2021. (18/13283-4, 18/15549-1, 14/06863-3, 17/03966-4, 15/26722-8, 17/19854-0, 17/04032-5, 18/18257-1)

MULE, SIMON NGAO; ROSA-FERNANDES, LIVIA; COUTINHO, JOAO V. P.; GOMES, VINICIUS DE MORAIS; MACEDO-DA-SILVA, JANAINA; SANTIAGO, VERONICA FEIJOLI; QUINA, DANIEL; DE OLIVEIRA, GILBERTO SANTOS; THAYSEN-ANDERSEN, MORTEN; LARSEN, MARTIN R.; et al. Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay. JOURNAL OF PROTEOMICS, v. 248, SEP 30 2021. (18/15549-1, 13/07937-8, 20/04923-0, 17/04032-5, 18/18257-1)

MATTOS, ELICIANE CEVOLANI; PALMISANO, GIUSEPPE; GOLDMAN, GUSTAVO H.. Phosphoproteomics of Aspergillus fumigatus Exposed to the Antifungal Drug Caspofungin. MSPHERE, v. 5, n. 3, p. 16-pg., 2020-05-01. (18/15549-1, 18/18257-1, 17/19288-5, 16/07870-9, 14/06863-3)

MESQUITA, JULIANA TONINI; MOURA, GABRIEL CANDIDO; CAVALCANTE, TAINA; YOKOYAMA YASUNAKA, JENICER KAZUMI UMADA; PERES, MANOEL APARECIDO; BLANES ANGELI, CLAUDIA; TANIWAKI, NOEMI NOSOMI; SIMONSEN STOLF, BEATRIZ; PALMISANO, GIUSEPPE; KATZIN, ALEJANDRO MIGUEL. In vitro and in vivo study of the inhibition of ubiquinone biosynthesis in Leishmania (Leishmania) amazonensis using the combination of buparvaquone and 4-nitrobenzoate as a strategy for the discovery of new therapeutic targets. Antimicrobial Agents and Chemotherapy, v. N/A, p. 17-pg., 2025-10-07. (24/09997-2, 17/22452-1, 20/04923-0, 21/01877-0, 18/15549-1)

LATROFA, MARIA STEFANIA; PALMISANO, GIUSEPPE; ANNOSCIA, GIADA; PIERRI, CIRO LEONARDO; CHANDRASHEKAR, RAMASWAMY; OTRANTO, DOMENICO. Major antigen and paramyosin proteins as candidate biomarkers for serodiagnosis of canine infection by zoonotic Onchocerca lupi. PLoS Neglected Tropical Diseases, v. 15, n. 2, p. 23-pg., 2021-02-01. (14/06863-3, 18/15549-1, 17/04032-5, 18/18257-1)

CARNEIRO, ANDREIA; MACEDO-DA-SILVA, JANAINA; SANTIAGO, VERONICA FEIJOLI; DE OLIVEIRA, GILBERTO SANTOS; GUIMARAES, THIAGO; MENDONCA, CLARISSA FEROLLA; DE OLIVEIRA BRANQUINHO, JESSICA LAIS; LUCENA, CINTIA VERDAN; OSORIO, JULIANA; PERNAMBUCO, EDUARDO; et al. Urine proteomics as a non-invasive approach to monitor exertional rhabdomyolysis during military training. JOURNAL OF PROTEOMICS, v. 258, p. 17-pg., 2022-04-30. (18/13283-4, 14/27198-8, 18/18257-1, 20/04923-0, 18/15549-1, 21/00140-3)

MULE, SIMON NGAO; GOMES, VINICIUS DE MORAIS; WAILEMANN, ROSANGELA A. M.; MACEDO-DA-SILVA, JANAINA; ROSA-FERNANDES, LIVIA; LARSEN, MARTIN R.; LABRIOLA, LETICIA; PALMISANO, GIUSEPPE. HSPB1 influences mitochondrial respiration in ER-stressed beta cells. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1869, n. 9, SEP 2021. (17/04032-5, 14/02745-6, 17/03618-6, 18/18257-1, 16/04676-7, 20/04923-0, 16/14845-0, 13/07937-8, 18/15549-1, 21/00507-4)

CARNEIRO, ANDREIA; VIANA-GOMES, DIEGO; MACEDO-DA-SILVA, JANAINA; OLIVEIRA LIMA, GISCARD HUMBERTO; MITRI, SIMONE; ALVES, SERGIO RABELLO; KOLLIARI-TURNER, ALEXANDER; ZANOTELI, EDMAR; DE AQUINO NETO, FRANCISCO RADLER; PALMISANO, GIUSEPPE; et al. Risk factors and future directions for preventing and diagnosing exertional rhabdomyolysis. Neuromuscular Disorders, v. 31, n. 7, p. 583-595, JUL 2021. (20/04923-0, 18/15549-1, 18/18257-1)

PINZAN, CAMILA FIGUEIREDO; VALERO, CLARA; DE CASTRO, PATRICIA ALVES; DA SILVA, JEFFERSON LUIZ; EARLE, KAYLEIGH; LIU, HONG; HORTA, MARIA AUGUSTA CRIVELENTE; KNIEMEYER, OLAF; KRUGER, THOMAS; PSCHIBUL, ANNICA; et al. Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation. NATURE MICROBIOLOGY, v. 9, n. 10, p. 30-pg., 2024-08-27. (22/13603-4, 22/08796-8, 20/04923-0, 18/18257-1, 18/15549-1, 21/04977-5)

CAVALCANTE, TAINA; MEDEIROS, MARIANA MEDINA; MULE, SIMON NGAO; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. The Role of Sialic Acids in the Establishment of Infections by Pathogens, With Special Focus on Leishmania. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, MAY 13 2021. (17/04032-5, 18/18257-1, 18/15549-1, 18/14972-8)

KAWAHARA, REBECA; RECUERO, SAULO; SROUGI, MIGUEL; LEITE, KATIA R. M.; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. The Complexity and Dynamics of the Tissue Glycoproteome Associated With Prostate Cancer Progression. MOLECULAR & CELLULAR PROTEOMICS, v. 20, 2021. (14/06863-3, 15/02866-0, 17/03010-8, 18/15549-1, 18/18257-1)

SUMAM DE OLIVEIRA, DAFFINY; KRONENBERGER, THALES; PALMISANO, GIUSEPPE; WRENGER, CARSTEN; DE SOUZA, EDMARCIA ELISA. Targeting SUMOylation in Plasmodium as a Potential Target for Malaria Therapy. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JUN 10 2021. (18/10150-3, 20/04923-0, 19/26771-0, 20/12277-0, 17/03966-4, 18/18257-1, 18/15549-1, 15/26722-8)

DOMBROWSKI, JAMILLE GREGORIO; BARATEIRO, ANDRE; MACHADO PEIXOTO, ERIKA PAULA; CLAUDIO DA SILVA BARROS, ANDRE BOLER; DE SOUZA, RODRIGO MEDEIROS; CLARK, TAANE GREGORY; CAMPINO, SUSANA; WRENGER, CARSTEN; WUNDERLICH, GERHARD; PALMISANO, GIUSEPPE; et al. Adverse pregnancy outcomes are associated with Plasmodium vivax malaria in a prospective cohort of women from the Brazilian Amazon. PLoS Neglected Tropical Diseases, v. 15, n. 4, APR 2021. (17/03966-4, 17/03939-7, 18/18257-1, 19/12068-5, 15/26722-8, 17/24267-7, 20/06747-4, 18/20468-0, 20/04923-0, 18/15549-1, 17/05782-8)

KISCHKEL, BRENDA; BONICHE-ALFARO, CAMILA; MENEZES, ISABELA DE GODOY; ROSSI, SUELEN ANDREIA; ANGELI, CLAUDIA BLANES; DE ALMEIDA, SANDRO ROGERIO; PALMISANO, GIUSEPPE; LOPES-BEZERRA, LEILA; NOSANCHUK, JOSHUA D.; TABORDA, CARLOS PELLESCHI. Immunoproteomic and Immunopeptidomic Analyses of Histoplasma capsulatum Reveal Promiscuous and Conserved Epitopes Among Fungi With Vaccine Potential. FRONTIERS IN IMMUNOLOGY, v. 12, NOV 22 2021. (18/18257-1, 16/08730-6, 14/06863-3, 17/25780-0, 18/26402-1, 18/15549-1)

TANO, FABIA TOMIE; BARBOSA, GUSTAVO ROLIM; DE REZENDE, ELOIZA; OLIVEIRA SOUZA, RODOLPHO ORNITZ; MUXEL, SANDRA MARCIA; SILBER, ARIEL MARIANO; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteome and morphological analysis show unexpected differences between promastigotes of Leishmania amazonensis PH8 and LV79 strains. PLoS One, v. 17, n. 8, p. 26-pg., 2022-08-23. (18/15549-1, 18/18257-1, 18/14972-8, 17/26197-6, 14/06863-3)

MULE, SIMON NGAO; SAAD, JOYCE SILVA; FERNANDES, LIVIA ROSA; STOLF, BEATRIZ S.; CORTEZ, MAURO; PALMISANO, GIUSEPPE. Protein glycosylation inLeishmaniaspp.. MOLECULAR OMICS, v. 16, n. 5, p. 407-424, OCT 1 2020. (20/04923-0, 18/15549-1, 16/23689-2, 17/04032-5)

FLOREZ-ALVAREZ, LIZDANY; DE SOUZA, EDMARCIA ELISA; BOTOSSO, VIVIANE FONGARO; LEAL DE OLIVEIRA, DANIELLE BRUNA; HO, PAULO LEE; TABORDA, CARLOS PELLESCHI; PALMISANO, GIUSEPPE; CAPURRO, MARGARETH LARA; REBELLO PINHO, JOAO RENATO; FERREIRA, HELENA LAGE; et al. Hemorrhagic fever viruses: Pathogenesis, therapeutics, and emerging and re-emerging potential. FRONTIERS IN MICROBIOLOGY, v. 13, p. 16-pg., 2022-10-25. (16/20045-7, 19/12303-4, 18/18257-1, 20/04923-0, 17/03966-4, 17/27131-9, 22/01812-8, 18/15549-1, 15/26722-8, 20/12277-0, 20/06409-1, 16/08730-6)

RIBEIRO, GIOVANE DE JESUS GOMES; YAN, SUN LIU REI; PALMISANO, GIUSEPPE; WRENGER, CARSTEN. Plant Extracts as a Source of Natural Products with Potential Antimalarial Effects: An Update from 2018 to 2022. PHARMACEUTICS, v. 15, n. 6, p. 19-pg., 2023-06-01. (18/15549-1, 18/18257-1, 20/04923-0, 17/26358-0, 15/26722-8)

TAVARES, MAURICIO T.; KRUEGER, ARNE; YAN, SUN L. REI; WAITMAN, KAROLINE B.; GOMES, VINICIUS M.; DE OLIVEIRA, DAFFINY SUMAM; PAZ, FRANCIARLI; HILSCHER, SEBASTIAN; SCHUTKOWSKI, MIKE; SIPPL, WOLFGANG; et al. 1,3-Diphenylureido hydroxamate as a promising scaffold for generation of potent antimalarial histone deacetylase inhibitors. SCIENTIFIC REPORTS, v. 13, n. 1, p. 20-pg., 2023-11-30. (18/08820-0, 18/10150-3, 20/04923-0, 21/00124-8, 18/18257-1, 21/08260-8, 15/26722-8, 18/15549-1, 22/12468-6, 17/26358-0, 21/11606-3, 17/03966-4, 22/07275-4)

ROSA-FERNANDES, LIVIA; SANTIAGO, VERONICA FEIJOLI; SILVA-SANTOS, YASMIN DA; LOPES, TISSIANE TAROSSO; PEIXOTO, ERIKA PAULA MACHADO; RODRIGUES, STEFANI APARECIDA MINCHIO; MARINHO, CLAUDIO ROMERO FARIAS; PALMISANO, GIUSEPPE; EPIPHANIO, SABRINA. Serum Proteomics of Experimental Malaria-Associated ARDS Reveals a Regulation of Acute-Phase Response Proteins. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2025, n. 1, p. 13-pg., 2025-01-01. (20/03163-1, 18/15549-1, 22/08582-8, 20/04923-0, 20/06747-4, 23/05478-8, 22/13150-0, 18/20468-0)

MARTINS, LARISSA ALMEIDA; PALMISANO, GIUSEPPE; CORTEZ, MAURO; KAWAHARA, REBECA; DE FREITAS BALANCO, JOSE MARIO; FUJITA, ANDRE; ALONSO, BEATRIZ IGLESIAS; BARROS-BATTESTI, DARCI MORAES; BRAZ, GLORIA REGINA CARDOSO; TIRLONI, LUCAS; et al. The intracellular bacterium Rickettsia rickettsii exerts an inhibitory effect on the apoptosis of tick cells. PARASITES & VECTORS, v. 13, n. 1, DEC 1 2020. (12/24105-3, 18/15549-1, 18/18257-1, 14/11513-1, 14/06863-3, 14/05855-7, 13/26450-2)

LAZARI, LUCAS C.; DE OLIVEIRA, GILBERTO SANTOS; MACEDO-DA-SILVA, JANAINA; ROSA-FERNANDES, LIVIA; PALMISANO, GIUSEPPE. Mass spectrometry and machine learning in the identification of COVID-19 biomarkers. FRONTIERS IN ANALYTICAL SCIENCE, v. 3, p. 15-pg., 2023-03-31. (20/04923-0, 18/18257-1, 21/00140-3, 18/15549-1, 18/13283-4)

KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, SI, NOV 2019. (18/15549-1, 15/02866-0, 18/18257-1, 17/03010-8, 14/06863-3)

KAWAHARA, REBECA; CHERNYKH, ANASTASIA; ALAGESAN, KATHIRVEL; BERN, MARSHALL; CAO, WEIQIAN; CHALKLEY, ROBERT J.; CHENG, KAI; CHOO, MATTHEW S.; EDWARDS, NATHAN; GOLDMAN, RADOSLAV; et al. Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis. NATURE METHODS, v. 18, n. 11, p. 1304+, NOV 2021. (18/15549-1)

MULE, SIMON NGAO; COSTA-MARTINS, ANDRE GUILHERME; ROSA-FERNANDES, LIVIA; DE OLIVEIRA, GILBERTO SANTOS; RODRIGUES, CARLA MONADELI F.; QUINA, DANIEL; ROSEIN, GRAZIELLA E.; GERALDES TEIXEIRA, MARTA MARIA; PALMISANO, GIUSEPPE. PhyloQuant approach provides insights into Trypanosoma cruzi evolution using a systems-wide mass spectrometry-based quantitative protein profile. COMMUNICATIONS BIOLOGY, v. 4, n. 1, MAR 11 2021. (18/13283-4, 17/04032-5, 14/06863-3, 18/15549-1, 18/18257-1, 14/25494-9)

MOREIRA, RAFAEL J.; CASTRO, ERIQUE; OLIVEIRA, TIAGO E.; BELCHIOR, THIAGO; PEIXOTO, ALBERT S.; CHAVES-FILHO, ADRIANO B.; MORENO, MAYARA F.; LIMA, JANAYNA D.; YOSHINAGA, MARCOS; MIYAMOTO, SAYURI; et al. Lipoatrophy-Associated Insulin Resistance and Hepatic Steatosis are Attenuated by Intake of Diet Rich in Omega 3 Fatty Acids. MOLECULAR NUTRITION & FOOD RESEARCH, v. 64, n. 7, FEB 2020. (13/07467-1, 16/04000-3, 17/17943-6, 15/19530-5, 14/06863-3, 18/15549-1, 13/07937-8)

LIMA, GUILHERME MEIRA; EFFER, BRIAN; BIASOTO, HENRIQUE PELLIN; FEIJOLI, VERONICA; PESSOA, ADALBERTO; PALMISANO, GIUSEPPE; MONTEIRO, GISELE. Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis. Biochemical Engineering Journal, v. 156, APR 15 2020. (15/07749-2, 13/08617-7, 14/06863-3, 18/18257-1, 18/15549-1, 16/15787-4)

SOUZA JUNIOR, DOUGLAS RICARDO; MARTINS SILVA, AMANDA RIBEIRO; ROSA-FERNANDES, LIVIA; REIS, LORENNA ROCHA; ALEXANDRIA, GABRIELLY; BHOSALE, SANTOSH D.; GHILARDI, FABIO DE ROSE; DALCOQUIO, TALIA FALCAO; BERTOLIN, ADRIADNE JUSTI; NICOLAU, JOSE CARLOS; et al. HDL proteome remodeling associates with COVID-19 severity. JOURNAL OF CLINICAL LIPIDOLOGY, v. 15, n. 6, p. 796-804, NOV-DEC 2021. (15/26722-8, 16/00696-3, 20/04923-0, 19/25702-4, 17/07725-1, 18/15549-1, 18/18257-1, 13/07937-8)

MISERANI MAGALHAES, RUBENS DANIEL; MATTOS, ELICIANE CEVOLANI; ROZANSKI, ANDREI; FAVORETTO GALANTE, PEDRO ALEXANDRE; PALMISANO, GIUSEPPE; CRUZ, ANGELA KAYSEL; COLLI, WALTER; CAMARGO, ANAMARIA ARANHA; MANSO ALVES, MARIA JULIA. Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix. PLoS Neglected Tropical Diseases, v. 14, n. 5, MAY 2020. (14/10046-0, 13/16002-2, 18/15549-1, 18/18257-1, 14/25494-9, 14/06863-3)

MACEDO-DA-SILVA, JANAINA; SANTIAGO, VERONICA F.; ROSA-FERNANDES, LIVIA; MARINHO, CLAUDIO R. F.; PALMISANO, GIUSEPPE. Protein glycosylation in extracellular vesicles: Structural characterization and biological functions. Molecular Immunology, v. 135, p. 226-246, JUL 2021. (18/15549-1, 18/20468-0, 18/18257-1, 20/04923-0)

KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, p. 12-pg., 2019-10-20. (17/03010-8, 18/18257-1, 18/15549-1, 14/06863-3, 15/02866-0)

LATROFA, MARIA STEFANIA; PALMISANO, GIUSEPPE; ANNOSCIA, GIADA; PIERRI, CIRO LEONARDO; CHANDRASHEKAR, RAMASWAMY; OTRANTO, DOMENICO. Major antigen and paramyosin proteins as candidate biomarkers for serodiagnosis of canine infection by zoonotic Onchocerca lupi. PLoS Neglected Tropical Diseases, v. 15, n. 2, FEB 2021. (17/04032-5, 18/18257-1, 14/06863-3, 18/15549-1)

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