| Grant number: | 19/06372-3 |
| Support Opportunities: | Multi-user Equipment Program |
| Start date: | August 01, 2019 |
| End date: | August 31, 2026 |
| Field of knowledge: | Biological Sciences - Immunology - Cellular Immunology |
| Principal Investigator: | Alessandro dos Santos Farias |
| Grantee: | Alessandro dos Santos Farias |
| Host Institution: | Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| City of the host institution: | Campinas |
| Associated researchers: | José Luiz Proença Módena ; Marcelo Alves da Silva Mori ; Marco Aurélio Ramirez Vinolo ; Pedro Manoel Mendes de Moraes Vieira |
| As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável | |
| EMU web page: | Página do Equipamento Multiusuário não informada |
| Type of equipment: | Processos Biológicos - Caracterização - Contagem células (inclui citômetros) |
| Manufacturer: | Fabricante não informado |
| Model: | Modelo não informado |
Abstract
Flow cytometry is a technology that provides the analysis of physical and chemical characteristics of a particular suspended particle. Flow cytometry allows the detection of multiple simultaneous characteristics (size, complexity and fluorescence intensity) of an individualized particle or cell. After the advent of monoclonal antibodies, the main application of flow cytometry was the identification of cell populations and subpopulations through surface and intracellular factors. In the last decades, several technologies were developed providing other applications for flow cytometry, which include the determination of solutes, identification of cell cycle phases, apoptosis, cell proliferation, identification of bacterial and viral particles, expression and protein phosphorylation (PhosFlow) and identification and quantification of gene expression (PrimeFlow). More recently, the development of equipments capable of analyzing simultaneously several parameters (> 20) provided a new era in flow cytometry. Today, multiparametric cytometry is the state of the art in immunology research. This technology has enabled the identification of new cellular populations. The possibility of analyzing up to 50 parameters in a single cell not only alters the discriminatory capacity of cellular subtypes, but also provides greater data reliability and faster data generation. Not least, the increased analytical capacity of a single experiment provides considerable savings in reagents and materials, which optimize the expense of resources. (AU)
| Articles published in Agência FAPESP Newsletter about the research grant: |
| More itemsLess items |
| TITULO |
| Articles published in other media outlets ( ): |
| More itemsLess items |
| VEICULO: TITULO (DATA) |
| VEICULO: TITULO (DATA) |