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Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA)

Grant number: 19/13496-0
Support type:Multi-user Equipment Program
Duration: August 01, 2019 - July 31, 2026
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Maurício Ariel Rostagno
Grantee:Maurício Ariel Rostagno
Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Associated research grant:18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Tipo de equipamento:Caracterização de Materiais - Análises Químicas - Cromatrografia líquida
Fabricante: Fabricante não informado
Modelo: Modelo não informado

Abstract

This research project proposes the development of a two-dimensional extraction, separation and analysis system (PLEgrad-SPE x UHPLC) by coupling extraction with gradient pressurized liquids (PLEgrad) with solid phase extraction (SPE) in the first dimension and orthogonally with ultra-high pressure chromatography (UHPLC) in the second dimension. The objective is to develop a system that allows the characterization of the chemical composition of natural raw materials in a fast, efficient and with real-time monitoring, while allowing the production of small quantities of extracts obtained through the fractionation of the present compounds. These fractions should be well characterized chemically and with low contamination between them. The system will be validated with several samples, including soybean (Glycine max), Brazilian ginseng (Pfaffia glomerata), black tea and green tea (Camellia sinensis), mate (Ilex paraguariensis), coffee (Coffea arabica), pomegranate granatum), orange peel (Citrus sinensis), sicilian lemon peel (Citrus x limon) apple peel (Malus domestica), jabuticaba bark (Myrciaria cauliflora), rosemary (Rosmarinus officinalis) and sage (Salvia officinalis). Initially the rapid UHPLC analysis methods will be developed that will be used in the second separation dimension. Several analysis conditions (temperature, column size, flow, volume and injection solvent and mobile phase composition) will be evaluated for an ultra-fast separation method (time between samples <2.0 min). Subsequently, different extraction conditions will be evaluated by SPE coupled gradient PLE, including temperature, pressure, sample amount, adsorbent type, flow, solvent and duration of the extraction / elution gradient. This proposal consists of a continuation of the Young Researcher project (FAPESP 2013 / 04304-4), seeking to consolidate this line of research at FCA / UNICAMP and to continue the training of qualified human resources. (AU)