Nuclei and chromatin alterations through cell cycle and senescence in mammalian cells

• Grant number: 11/22619-7
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: April 01, 2012
• End date: March 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Julia Pinheiro Chagas da Cunha
• Grantee: Julia Pinheiro Chagas da Cunha
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Fábio Nakano ; Paulo Costa Carvalho ; Pedro Alexandre Favoretto Galante
• Associated research grant(s): 18/15553-9 - Going deeper into Trypanosoma cruzi chromatin regulation: identifying new players and quizzing its impact on a potential transcription control, AP.JP2
• Associated scholarship(s): 16/24881-4 - Nuclear proteome alterations induced by growth factor along cell cycle in mice tumour cells, BP.DD; 15/14522-4 - Analysis of FosB and JunB expression and interaction partners after stimulation of FGF-2 tumor cells, BP.IC; 15/04867-4 - Nuclear proteome alterations induced by growth factor along cell cycle in mice tumour cells, BP.DD

Abstract

The nucleus is a complex organelle composed of nucleic acids and proteins in a tight relationship. The nuclear proteome, specially the chromatin proteome, plays a role in the regulation and management of genome that ultimately controls gene expression and cell phenotype. Many evidences demonstrate an important role of nucleus and chromatin in eukaryotic cell cycle control. The fibroblast growth factor 2 transiently blocks mice tumor cells in G1àS transition followed by irreversible blockage in G2à M. At this latter point, it is possible to detect cells with a senescent phenotype. Thus, the main objective of this project is to study the role of nucleus and chromatin proteome in cell cycle and senescence induced by FGF2. For this purpose, we will establish a platform of quantitative proteomic with special emphasis on the study of cell nucleus and chromatin with a Systems Biology perspective of data analysis and interpretation. We intend to analyze in more detail the pattern of histone post translational modifications through cell cycle and senescence in order to investigate if they can play a role in cell cycle or senescence progression. The dynamic of protein-DNA interactions will be also investigated and integrated with the proteomic alterations in order to develop a complete and systemic view of FGF2 effects in nucleus. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LUND, PEDER J.; LOPES, MARIANA; SIDOLI, SIMONE; CORADIN, MARIEL; DE LUNA VITORINO, FRANCISCA NATHALIA; CHAGAS DA CUNHA, JULIA PINHEIRO; GARCIA, BENJAMIN A.. FGF-2 induces a failure of cell cycle progression in cells harboring amplified K-Ras, revealing new insights into oncogene-induced senescence. MOLECULAR OMICS, v. 17, n. 5, p. 725-739, OCT 11 2021. (16/24881-4, 15/04867-4, 13/07467-1, 17/15835-1, 18/15553-9, 11/22619-7, 17/18344-9)

DE LUNA VITORINO, FRANCISCA NATHALIA; MONTONI, FABIO; MORENO, JAQUELINE NEVES; DE SOUZA, BRUNO FERREIRA; LOPES, MARIANA DE CAMARGO; CORDEIRO, BARBARA; FONSECA, CECILIA SELLA; GILMORE, JOSHUA M.; SARDIU, MIHAELA I.; REIS, MARCELO SILVA; et al. FGF2 Antiproliferative Stimulation Induces Proteomic Dynamic Changes and High Expression of FOSB and JUNB in K-Ras-Driven Mouse Tumor Cells. PROTEOMICS, v. 18, n. 17, SEP 2018. (11/22619-7, 13/07467-1)

LEANDRO DE JESUS, TERESA CRISTINA; CALDERANO, SIMONE GUEDES; DE LUNA VITORINO, FRANCISCA NATHALIA; LLANOS, RICARDO PARIONA; LOPES, MARIANA DE CAMARGO; DE ARAUJO, CHRISTIANE BEZERRA; THIEMANN, OTAVIO HENRIQUE; REIS, MARCELO DA SILVA; ELIAS, MARIA CAROLINA; CHAGAS DA CUNHA, JULIA PINHEIRO. Quantitative Proteomic Analysis of Replicative and Nonreplicative Forms Reveals Important Insights into Chromatin Biology of Trypanosoma cruzi. MOLECULAR & CELLULAR PROTEOMICS, v. 16, n. 1, p. 23-38, JAN 2017. (13/07467-1, 11/22619-7)

LUND, PEDER J.; LOPES, MARIANA; SIDOLI, SIMONE; CORADIN, MARIEL; DE LUNA VITORINO, FRANCISCA NATHALIA; CHAGAS DA CUNHA, JULIA PINHEIRO; GARCIA, BENJAMIN A.. FGF-2 induces a failure of cell cycle progression in cells harboring amplified K-Ras, revealing new insights into oncogene-induced senescence. MOLECULAR OMICS, JUN 2021. (11/22619-7, 16/24881-4, 17/15835-1, 18/15553-9, 15/04867-4, 17/18344-9, 13/07467-1)

LEANDRO DE JESUS, TERESA CRISTINA; NUNES, VINICIUS SANTANA; LOPES, MARIANA DE CAMARGO; MARTIL, DAIANA EVELIN; IWAI, LEO KEI; MORETTI, NILMAR SILVIO; MACHADO, FABRICIO CASTRO; DE LIMA-STEIN, MARIANA L.; THIEMANN, OTAVIO HENRIQUE; ELIAS, MARIA CAROLINA; et al. Chromatin Proteomics Reveals Variable Histone Modifications during the Life Cycle of Trypanosoma cruzi. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 6, p. 2039-2051, JUN 2016. (13/07467-1, 14/03714-7, 08/57910-0, 15/04867-4, 11/06087-5, 11/51973-3, 11/22619-7, 14/01577-2)