Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system

• Grant number: 15/19890-1
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2015
• End date: November 30, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Ronaldo Censi Faria
• Grantee: Ronaldo Censi Faria
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

This project proposes the development of analytical methods for the determination of protein biomarkers for the diagnosis of diseases as cancer and Alzheimer's. To achieve this goal a new full disposable microfluidic device to work with eight to twelve independent carbon electrode will be developed that will allow the multiplexed detection of protein biomarkers. This device will be constructed using low-cost equipment and materials, and it will be used simple microfluidic system using a syringe pump and a chromatographic valve for the sample injection. The analytical methods will be developed using magnetic particles heavily decorated with primary antibody and enzymatic labels for capture, separation, preconcentration of the analytes and signal amplification. With this, we expected to develop analytical methods for multiplexed determination of biomarkers with very low limit of detection, high selectivity and low-cost. The project has also the objective to produce papers in high-impact journals and primarily contribute to formation of high quality human resources for research. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, TASSIA REGINA; MARTUCCI, DIEGO HENRIQUE; FARIA, RONALDO CENSI. Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay. SENSORS AND ACTUATORS B-CHEMICAL, v. 255, n. 1, p. 684-691, FEB 2018. (14/21115-3, 14/22401-0, 15/19890-1)

PROENCA, CAMILA A.; BALDO, THAISA A.; FREITAS, TAYANE A.; MATERON, ELSA M.; WONG, ADEMAR; DURAN, ANDRES A.; MELENDEZ, MATIAS E.; ZAMBRANO, GUSTAVO; FARIA, RONALDO C.. Novel enzyme-free immunomagnetic microfluidic device based on Co0.25Zn0.75Fe2O4 for cancer biomarker detection. Analytica Chimica Acta, v. 1071, p. 59-69, SEP 13 2019. (17/24053-7, 16/00991-5, 15/19890-1)

DE OLIVEIRA, RICARDO A. G.; CAMARGO, FIAMMA; PESQUERO, NAIRA C.; FARIA, RONALDO CENSI. A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis. Analytica Chimica Acta, v. 957, p. 40-46, MAR 8 2017. (15/19890-1)

DE OLIVEIRA, TASSIA R.; MANZINE, PATRICIA R.; COMINETTI, MARCIA R.; LEITE, OLDAIR D.; FARIA, RONALDO C.. Electrochemical magneto-immunoassay for detection of ADAM10 Alzheimer's biomarker using gold nanoparticles as label. Talanta, v. 266, p. 8-pg., 2024-01-01. (17/24053-7, 15/26084-1, 17/24839-0, 15/19890-1, 21/01863-9)

DE OLIVEIRA, TASSIA REGINA; MARTUCCI, DIEGO HENRIQUE; FARIA, RONALDO CENSI. Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay. SENSORS AND ACTUATORS B-CHEMICAL, v. 255, p. 8-pg., 2018-02-01. (14/21115-3, 14/22401-0, 15/19890-1)

DE OLIVEIRA, TASSIA R.; ERBERELI, CAMILA R.; MANZINE, PATRICIA R.; MAGALHAES, THAMIRES N. C.; BALTHAZAR, MARCIO L. F.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Early Diagnosis of Alzheimer's Disease in Blood Using a Disposable Electrochemical Microfluidic Platform. ACS SENSORS, v. 5, n. 4, p. 1010-1019, APR 24 2020. (14/22401-0, 15/26084-1, 18/15571-7, 17/24839-0, 17/24053-7, 15/19890-1)

FREITAS, TAYANE A.; PROENCA, CAMILA A.; BALDO, THAISA A.; MATERON, ELSA M.; WONG, ADEMAR; MAGNANI, RODRIGO F.; FARIA, RONALDO C.. Ultrasensitive immunoassay for detection of Citrus tristeza virus in citrus sample using disposable microfluidic electrochemical device. Talanta, v. 205, DEC 1 2019. (17/24053-7, 16/00991-5, 15/19890-1)

ULIANA, CAROLINA V.; DE OLIVEIRA, TASSIA R.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Label-free evaluation of small-molecule-protein interaction using magnetic capture and electrochemical detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 10, p. 2111-2119, APR 2019. (15/24940-8, 14/22401-0, 15/19890-1)

MATERON, ELSA MARIA; WONG, ADEMAR; FATIBELLO-FILHO, ORLANDO; FARIA, RONALDO CENSI. Development of a simple electrochemical sensor for the simultaneous detection of anticancer drugs. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 827, p. 64-72, OCT 15 2018. (15/19890-1)

DE OLIVEIRA, RICARDO A. G.; MATERON, ELSA M.; MELENDEZ, MATIAS E.; CARVALHO, ANDRE L.; FARIA, RONALDO C.. Disposable Microfluidic Immunoarray Device for Sensitive Breast Cancer Biomarker Detection. ACS APPLIED MATERIALS & INTERFACES, v. 9, n. 33, p. 27433-27440, AUG 23 2017. (15/19890-1)

DE OLIVEIRA, TASSIA REGINA; FONSECA, WILSON TIAGO; SETTI, GRAZIELLE DE OLIVEIRA; FARIA, RONALDO CENSI. Fast and flexible strategy to produce electrochemical paper-based analytical devices using a craft cutter printer to create wax barrier and screen-printed electrodes. Talanta, v. 195, p. 480-489, APR 1 2019. (17/24053-7, 14/22401-0, 15/19890-1)

ULIANA, CAROLINA V.; PEVERARI, CAMILA R.; AFONSO, ANDRE S.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Fully disposable microfluidic electrochemical device for detection of estrogen receptor alpha breast cancer biomarker. BIOSENSORS & BIOELECTRONICS, v. 99, p. 156-162, JAN 15 2018. (13/06750-1, 12/24079-2, 15/19890-1)

Filed patent(s) as a result of this research project