Adsorption of pollutants metals and enzymes in hybrid Langmuir-Blodgett films immobilized with algal polysaccharides / phospholipids

• Grant number: 11/14289-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2012
• End date: March 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Audrey Kalinouski de Brito
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated research grant: 08/10851-0 - Ultrathin films to fabrication of biosensors and biomembrane mimicking: applications in environmental and health areas, AP.JP

Abstract

In this project, we intend to study the action of exopolysaccharides from microalgae Cryptomonas tetrapirenoidosa in Langmuir-Blodgett (LB) films mounted with the lipid DODAB under several conditions to be made a biosensor capable of detecting and remove pollutants metals from aqueous solutions. The lipid is spread on the air-water interface, and the microalgae extract is injected into the aqueous subphase. So, the monolayer is transferred to a solid substrate resulting the LB film. These films will have their capacity to adsorb metals tested and characterized by spectroscopic and microscopic techniques. It will also be tested the possibility of fabrication of a urea sensor through the enzyme urease, whose affinity for microalgae extracts will be tested. The adsorption of the extracts will be evaluated through measures of pressure and surface potential, kinetic curves of adsorption, infrared interface, and atomic force microscopy. Results will be evaluated from the perspective of possible biological implications, inferred from the effects caused by the extracts in artificial membranes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE BRITO, AUDREY KALINOUSKI; NORDI, CRISTINA S. F.; CASELI, LUCIANO. Algal polysaccharides as matrices for the immobilization of urease in lipid ultrathin films studied with tensiometry and vibrational spectroscopy: Physical-chemical properties and implications in the enzyme activity. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 135, p. 639-645, NOV 1 2015. (11/14289-7, 13/10213-1)