Development and validation of methodologies for the determination of the antioxidant capacity of carotenoids against reactive oxygen and nitrogen species

• Grant number: 12/01356-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Effective date (Start): May 14, 2012
• Effective date (End): May 13, 2013
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Adriana Zerlotti Mercadante
• Grantee: Renan Campos Chisté
• Supervisor: Eduarda das Graças Rodrigues Fernandes
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: Universidade do Porto (UP), Portugal
• Associated to the scholarship: 11/15343-5 - Development and validation of methodologies for the determination of the antioxidant capacity of carotenoids against reactive oxygen and nitrogen species, BP.PD

Abstract

Carotenoids are natural fat-soluble pigments which have important properties for a variety of biological functions. They are related to the reduction or inhibition of oxidation reactions, through scavenging of reactive oxygen (ROS) and nitrogen (RNS) species overproduced by the organism. This project of "Intern Scholarship of Abroad Research" (BEPE) will be directed to the development and validation of methodologies by fluorescence, chemiluminescence or colorimetric method for determining the scavenging capacity of carotenoids against the main ROS and RNS of biological interest. These methodologies are scarce in the literature due to the fact that the major existing methods have been developed to evaluate the activity of hydrophilic compounds, or due to interference of carotenoids with the probes used for detection of reactive species, overestimating the fluorescence, chemiluminescence or colorimetric responses. The BEPE project will be executed in the Faculty of Pharmacy, University of Porto (Portugal), under the supervision of Prof. PhD Eduarda das Graças Rodrigues Fernandes. This proposal, including the partnership already confirmed, is described in the post-doctorate project granted by FAPESP (Proc. 2011/15343) in December 2011, resulting in the consolidation of the scientific partnership between both institutions (UNICAMP and University of Porto). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHISTE, RENAN CAMPOS; FREITAS, MARISA; MERCADANTE, ADRIANA ZERLOTTI; FERNANDES, EDUARDA. Carotenoids inhibit lipid peroxidation and hemoglobin oxidation, but not the depletion of glutathione induced by ROS in human erythrocytes. Life Sciences, v. 99, n. 1-2, p. 52-60, MAR 18 2014. (12/01356-0, 13/07914-8)