Joint association of fruit, vegetable, and heterocyclic amine intake with DNA damage levels in a general population

Author(s)

de Carvalho, Aline Martins ^{[1, 2]} ; Ferreira Carioca, Antonio Augusto ^[1] ; Fisberg, Regina Mara ^[1] ; Qi, Lu ^[2] ; Marchioni, Dirce Maria ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Publ Hlth, Sao Paulo - Brazil

^[2] Harvard Univ, Sch Publ Hlth, 665 Huntington Ave, Boston, MA 02115 - USA

• Document type: Journal article
• Source: NUTRITION; v. 32, n. 2, p. 260-264, FEB 2016.
• Web of Science Citations: 4

Abstract

Objective: To assess joint effects of heterocyclic amine (HCA), fruit, and vegetable intake on DNA damage in a general population. Methods: A cross-sectional survey (ISA-Capital) was performed among adults and older adults in Brazil. We selected 73 participants with high HCA intake and 73 sex- and age-matched participants with non-HCA intake (n = 146) for the present study. Diet was assessed by a 24-h dietary recall and a structured questionnaire with cooking methods and levels of meat doneness. DNA damage was measured by 8-oxo-2'-deoxyguanosine (8-OHdG). The association between DNA damage and dietary intake was analyzed by linear regression models. Results: Fruit intake showed significantly inverse association with 8-OHdG (beta, -0.787; P = 0.035), whereas HCA intake was significantly associated with increased DNA damage (beta, 1.621; P = 0.036) after adjusting for covariates, including sex, age, body mass index, energy intake, smoking, physical activity, and C-reactive protein. Vegetable intake was not significantly associated with 8-OHdG. We also found a significant association between joint fruit and HCA intake and DNA damage, and the difference in 8-OHdG levels was significantly higher between participants with the lowest fruit intake and highest HCA intake and those with the highest fruit intake and non-HCA intake (P = 0.049). Conclusions: Lower intake of fruits and higher intake of HCAs were associated with higher DNA damage levels and showed an additive effect pattern. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/04607-0 - Relationship between meat and heterocyclic amines intake and biochemical and genetic biomarkers
• Grantee: Aline Martins de Carvalho
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 14/16347-2 - Metabolic signature on migrants and their relationship to patterns of consumption and metabolic syndrome: an epidemiological approach to elucidate the effects of diet
• Grantee: Antonio Augusto Ferreira Carioca
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/10965-0 - Meat and heterocyclic amines intake, relationship with biomarkers for exposure and susceptibility to cancer in ISA-Capital study
• Grantee: Aline Martins de Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/15831-0 - Dietary factors, homocystein, MTHFR gene polymorphisms, and cardiovascular risk in adults and the elderly: a population-based study - ISA - Capital
• Grantee: Regina Mara Fisberg
• Support Opportunities: Regular Research Grants