Infraspecific Chemical Variability and Biological Activity of Casearia sylvestris from Different Brazilian Biomes

Author(s)

Bueno, Paula Carolina Pires ^[1] ; Salome Abarca, Luis Francisco ^[2] ; Anhesine, Naira Buzzo ^[1] ; Giffoni, Maira Silva ^[1] ; Pereira, Fabiola Manhas Verbi ^{[1, 3]} ; Torres, Roseli Buzaneli ^[4] ; de Sousa, Rayran Walter Ramos ^[5] ; Ferreira, Paulo Michel Pinheiro ^[5] ; Pessoa, Claudia ^[6] ; Cavalheiro, Alberto Jose ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Dept Biochem & Organ Chem, Inst Chem, Rua Prof Francisco Degni 55, BR-14800900 Araraquara, SP - Brazil

^[2] Leiden Univ, Inst Biol, Nat Prod Lab, Leiden - Netherlands

^[3] Natl Inst Alternat Technol Detect Toxicol Assessm, Araraquara, SP - Brazil

^[4] Herbarium IAC, Agron Inst Campinas, Campinas, SP - Brazil

^[5] Univ Fed Piaui, Lab Expt Cancerol, Dept Biophys & Physiol, Teresina, PI - Brazil

^[6] Univ Fed Ceara, Fac Med, Dept Physiol & Pharmacol, Fortaleza, CE - Brazil

• Document type: Journal article
• Source: Planta Medica; v. 87, n. 01/02 DEC 2020.
• Web of Science Citations: 0

Abstract

Casearia sylvestris is an outstanding representative of the Casearia genus. This representability comes from its distinctive chemical profile and pharmacological properties. This species is widespread from North to South America, occurring in all Brazilian biomes. Based on their morphology, 2 varieties are recognized: C. sylvestris var. sylvestris and C. sylvestris var. lingua . Despite the existence of data about their chemical composition, a deeper understanding of the specialized metabolism correlation and variation in respect to environmental factors and its repercussion over their biological activities was still pending. In this study, an UHPLC-DAD-based metabolomics approach was employed for the investigation of the chemical variation of 12 C. sylvestris populations sampled across 4 Brazilian biomes and ecotones. The correlation between infraspecific chemical variability and the cytotoxic and antioxidant activities was achieved by multivariate data analysis. The analyses showed that C. sylvestris var. lingua prevailed at Cerrado areas, and it was correlated with lower cytotoxic activity and high level of glycosylated flavonoids. Among them, narcissin and isorhamnetin-3- O-alpha -L-rhamnopyranosyl-(1 -> 2)- alpha -L-arabinopyranoside showed good correlation with the antioxidant activity. Conversely, C. sylvestris var. sylvestris prevailed at the Atlantic Forest areas, and it was associated with high cytotoxic activity and high content of clerodane diterpenoids. Different casearins showed good correlation ( R (2) =0.3-0.70) with the cytotoxic activity. These findings highlighted the great complexity among different C. sylvestris populations, their chemical profile, and the related biological activities. Consequently, it can certainly influence the medicinal properties, as well as the quality and efficacy, of C. sylvestris phytomedicines. (AU)

• FAPESP's process: 16/20295-3 - International PSE Symposium - Plant Omics and Biotechnology for Human Health
• Grantee: Paula Carolina Pires Bueno
• Support Opportunities: Research Grants - Meeting - Abroad
• FAPESP's process: 12/21921-4 - Proteomics of two varieties of Casearia sylvestris Sw. (Salicaceae)
• Grantee: Paula Carolina Pires Bueno
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 11/21440-3 - Infra-specific chemical variability and dynamics of phenolic and diterpenic secondary metabolites in Casearia sylvestris Sw (Salicaceae) from different Brazilian ecosystems: metabolomics and proteomics correlation
• Grantee: Paula Carolina Pires Bueno
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/01102-8 - Multi-user equipament approved in grant 2018/18212-8: X-Ray Fluorescence spectrometer
• Grantee: Fabiola Manhas Verbi Pereira
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/02738-0 - Chemical documentation of species from Casearia genus (Salicaceae) of ecological, economical and medicinal relevance
• Grantee: Naira Buzzo Anhesine
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/18212-8 - Development of chemometric models for determination of solid impurities in raw sugarcane using energy dispersive X-ray fluorescence
• Grantee: Fabiola Manhas Verbi Pereira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC