High-pressure microfluidization of whey proteins: Impact on protein structure and ability to bind and protect lutein

Author(s)

Vidotto, Danilo C. C. ; Mantovani, Raphaela A. A. ; Tavares, Guilherme M. M.

• Document type: Journal article
• Source: Food Chemistry; v. 382, p. 8-pg., 2022-07-15.

Abstract

Dynamic high-pressure homogenization microfluidization (DHPM) is a versatile emerging technology that may be applied to food processing to achieve several goals. DHPM may, depending on nature of the molecules and the working parameters, induce changes in protein structure, which may improve or impair their techno-functional properties and ability to bind other molecules. In this context, DHPM (12 passes, 120 MPa), coupled or not to a cooling device, was applied to 8-lactoglobulin (8-lg) and whey protein isolate (WPI) dispersions. Minor changes in the structure of whey proteins were induced by DHPM with sample cooling; although, when sample cooling was not applied, aggregation and increases of around 30 % of protein surface hydrophobicity were noticeable for the WPI dispersion. The association constant between the proteins and lutein was in the magnitude of 104 M-1, and lutein photodegradation constant diminished about 3 times in the presence of proteins, compared to in their absence. (AU)

• FAPESP's process: 17/09214-4 - How do structural changes of dairy proteins affect the functionality of bioactive compounds in complex food matrices?
• Grantee: Guilherme Miranda Tavares
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/23752-1 - Multi0user equipment approved in grant 2013/07914-8: high-speed liquid chromatography coupled to arrangement of diodes, fluorescence and masses
• Grantee: Adriana Zerlotti Mercadante
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 17/26237-8 - Understanding how protein-carotenoid interactions influence emulsion stability and carotenoid bioacessibility
• Grantee: Raphaela de Araujo Mantovani
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/09304-6 - Interaction between carotenoids and native and non-native globular proteins from milk: structural and functional effects in mono and multi protein systems
• Grantee: Danilo Corsi Vidotto
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/03822-5 - Multi-user equipment approved in grant 2017/09214-4: preparative chromatography system - Akta pure (Ge healthcare)
• Grantee: Guilherme Miranda Tavares
• Support Opportunities: Multi-user Equipment Program