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High-pressure microfluidization of whey proteins: Impact on protein structure and ability to bind and protect lutein

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Author(s):
Vidotto, Danilo C. C. ; Mantovani, Raphaela A. A. ; Tavares, Guilherme M. M.
Total Authors: 3
Document type: Journal article
Source: Food Chemistry; v. 382, p. 8-pg., 2022-07-15.
Abstract

Dynamic high-pressure homogenization microfluidization (DHPM) is a versatile emerging technology that may be applied to food processing to achieve several goals. DHPM may, depending on nature of the molecules and the working parameters, induce changes in protein structure, which may improve or impair their techno-functional properties and ability to bind other molecules. In this context, DHPM (12 passes, 120 MPa), coupled or not to a cooling device, was applied to 8-lactoglobulin (8-lg) and whey protein isolate (WPI) dispersions. Minor changes in the structure of whey proteins were induced by DHPM with sample cooling; although, when sample cooling was not applied, aggregation and increases of around 30 % of protein surface hydrophobicity were noticeable for the WPI dispersion. The association constant between the proteins and lutein was in the magnitude of 104 M-1, and lutein photodegradation constant diminished about 3 times in the presence of proteins, compared to in their absence. (AU)

FAPESP's process: 17/09214-4 - How do structural changes of dairy proteins affect the functionality of bioactive compounds in complex food matrices?
Grantee:Guilherme Miranda Tavares
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/23752-1 - Multi0user equipment approved in grant 2013/07914-8: high-speed liquid chromatography coupled to arrangement of diodes, fluorescence and masses
Grantee:Adriana Zerlotti Mercadante
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 17/26237-8 - Understanding how protein-carotenoid interactions influence emulsion stability and carotenoid bioacessibility
Grantee:Raphaela de Araujo Mantovani
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/09304-6 - Interaction between carotenoids and native and non-native globular proteins from milk: structural and functional effects in mono and multi protein systems
Grantee:Danilo Corsi Vidotto
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/03822-5 - Multi-user equipment approved in grant 2017/09214-4: preparative chromatography system - Akta pure (Ge healthcare)
Grantee:Guilherme Miranda Tavares
Support Opportunities: Multi-user Equipment Program