Simultaneous extraction and analysis of apple pomace by gradient pressurized liquid extraction coupled in-line with solid-phase extraction and on-line with HPLC

Author(s)

Da Silva, Laise C. ; Vigano, Juliane ; Sanches, Vitor L. ; De Souza Mesquita, Leonardo M. ; Pizani, Rodrigo ; Rostagno, Mauricio A.

• Document type: Journal article
• Source: Food Chemistry; v. 407, p. 11-pg., 2022-12-10.

Abstract

Due to the complex characteristics and variable composition of apple pomace, sample preparation for chro-matographic analysis is a great challenge. To solve this problem, we proposed using a solvent gradient using Pressurized Liquid Extraction (PLE), where the solvent gradually changes from water to ethanol during the extraction. Different dynamic gradients, static time, and temperatures were evaluated and showed relevant ef-fects on the yields of target analytes. It was possible to improve extraction yields of compounds with different characteristics using the extraction solvent gradient. By coupling solid-phase extraction in-line, it was possible to separate compounds into fractions, where furfural, HMF, and chlorogenic acid gradually eluted from the adsorbent. At the same time, flavonoids were retained and eluted in the later fractions. On-line analysis by HPLC provided real-time information about the process and permitted the creation of a 3D chromatogram of the sample. (AU)

• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 19/13496-0 - Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA)
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 19/24537-0 - Phytochemical evaluation of apple (Malus domestica) industrial residue from extraction and separation by a two-dimensional system
• Grantee: Laise Capelasso da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/04067-6 - Evaluation of ultrasound-assisted pressurized fluid extraction coupled with solid phase extraction for extracting phenolic compounds and terpenes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis)
• Grantee: Rodrigo Stein Pizani
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/15774-5 - Development of methods of extraction, separation and two-dimensional analysis of phenolic compounds in natural products
• Grantee: Juliane Viganó
• Support Opportunities: Scholarships in Brazil - Post-Doctoral