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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comprehensive analysis of phenolic compounds from natural products: Integrating sample preparation and analysis

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Author(s):
Vigano, Juliane [1] ; Sanches, Vitor L. [1] ; Mesquita, Leonardo M. de Souza [1] ; de Souza, Mariana C. [1] ; da Silva, Laise C. [1] ; Chaves, Jaisa O. [1] ; Forster-Carneiro, Tania [2] ; Rostagno, Mauricio A. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Sch Appl Sci FCA, Multidisciplinary Lab Food & Hlth LabMAS, Rua Pedro Zaccaria 1300, BR-13484350 Sao Paulo - Brazil
[2] Univ Campinas FEA UNICAMP, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Analytica Chimica Acta; v. 1178, SEP 15 2021.
Web of Science Citations: 0
Abstract

The comprehensive analysis of phenolic compounds from natural products comprises critical steps, including quantitative extraction, extract preparation, and chromatographic procedure. Performing these steps off-line requires a long time to obtain results, besides being laborious and more error-prone. This work discusses the concept and presents the details of assembling and validating a new system to comprehensively analyze phenolic compounds in natural products. The system is based on a bidimen-sional separation through the combination of pressurized liquid extraction with in-line solid-phase extraction coupled online with HPLC-PDA. The system proved to be able to perform a bidimensional separation to characterize the sample and ensure quantitative extraction of all detected components using the most appropriate extraction solvent gradient depending on the raw sample analyzed. The 1st dimension separation is achieved by PLE-SPE with a solvent gradient and differential interactions of extracted compounds with the adsorbent. The 2nd dimension presents the HPLC-PDA separation. The extraction/separation process can be monitored in real-time, and kinetic extraction curves for individual compounds can also be obtained to ensure quantitative extraction. Thus, the 2D PLE-SPE x HPLC-PDA may provide fast and precise comprehensive analyses of a large plethora of phenolic compounds, finding relevant applications in the chemical, food, pharmaceutical, and agricultural fields. (c) 2021 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 19/24537-0 - Phytochemical evaluation of apple (Malus domestica) industrial residue from extraction and separation by a two-dimensional system
Grantee:Laise Capelasso da Silva
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
Grantee:Maurício Ariel Rostagno
Support type: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 19/18772-6 - Development of fast methods for analysis and characterization chemical profile of compounds in black tea and mate leaves
Grantee:Mariana Corrêa de Souza
Support type: Scholarships abroad - Research Internship - Doctorate (Direct)
FAPESP's process: 20/08421-9 - Application of non-volatile alternative solvents to create sustainable two-dimensional and integrated platforms for harnessing agro-industrial waste from jaboticaba and pomegranate fruits
Grantee:Leonardo Mendes de Souza Mesquita
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/17089-8 - Separation of bioactive compounds from black tea and mate by on-line coupling of pressurized liquid extraction with an adsorbent and evaluation of their antimicrobial potential
Grantee:Mariana Corrêa de Souza
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology
Grantee:Maurício Ariel Rostagno
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 18/14938-4 - Waste2energy: integration of supercritical technologies and anaerobic digestion through an automated system for the production of biofuel precursors
Grantee:Tânia Forster Carneiro
Support type: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 20/15774-5 - Development of methods of extraction, separation and two-dimensional analysis of phenolic compounds in natural products
Grantee:Juliane Viganó
Support type: Scholarships in Brazil - Post-Doctorate