Comprehensive analysis of phenolic compounds from natural products: Integrating sample preparation and analysis

Author(s)

Vigano, Juliane ^[1] ; Sanches, Vitor L. ^[1] ; Mesquita, Leonardo M. de Souza ^[1] ; de Souza, Mariana C. ^[1] ; da Silva, Laise C. ^[1] ; Chaves, Jaisa O. ^[1] ; Forster-Carneiro, Tania ^[2] ; Rostagno, Mauricio A. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Appl Sci FCA, Multidisciplinary Lab Food & Hlth LabMAS, Rua Pedro Zaccaria 1300, BR-13484350 Sao Paulo - Brazil

^[2] Univ Campinas FEA UNICAMP, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Analytica Chimica Acta; v. 1178, SEP 15 2021.
• Web of Science Citations: 0

Abstract

The comprehensive analysis of phenolic compounds from natural products comprises critical steps, including quantitative extraction, extract preparation, and chromatographic procedure. Performing these steps off-line requires a long time to obtain results, besides being laborious and more error-prone. This work discusses the concept and presents the details of assembling and validating a new system to comprehensively analyze phenolic compounds in natural products. The system is based on a bidimen-sional separation through the combination of pressurized liquid extraction with in-line solid-phase extraction coupled online with HPLC-PDA. The system proved to be able to perform a bidimensional separation to characterize the sample and ensure quantitative extraction of all detected components using the most appropriate extraction solvent gradient depending on the raw sample analyzed. The 1st dimension separation is achieved by PLE-SPE with a solvent gradient and differential interactions of extracted compounds with the adsorbent. The 2nd dimension presents the HPLC-PDA separation. The extraction/separation process can be monitored in real-time, and kinetic extraction curves for individual compounds can also be obtained to ensure quantitative extraction. Thus, the 2D PLE-SPE x HPLC-PDA may provide fast and precise comprehensive analyses of a large plethora of phenolic compounds, finding relevant applications in the chemical, food, pharmaceutical, and agricultural fields. (c) 2021 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 19/24537-0 - Phytochemical evaluation of apple (Malus domestica) industrial residue from extraction and separation by a two-dimensional system
• Grantee: Laise Capelasso da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 19/18772-6 - Development of fast methods for analysis and characterization chemical profile of compounds in black tea and mate leaves
• Grantee: Mariana Corrêa de Souza
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 20/08421-9 - Application of non-volatile alternative solvents to create sustainable two-dimensional and integrated platforms for harnessing agro-industrial waste from jaboticaba and pomegranate fruits
• Grantee: Leonardo Mendes de Souza Mesquita
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/17089-8 - Separation of bioactive compounds from black tea and mate by on-line coupling of pressurized liquid extraction with an adsorbent and evaluation of their antimicrobial potential
• Grantee: Mariana Corrêa de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/14938-4 - Waste2energy: integration of supercritical technologies and anaerobic digestion through an automated system for the production of biofuel precursors
• Grantee: Tânia Forster Carneiro
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 20/15774-5 - Development of methods of extraction, separation and two-dimensional analysis of phenolic compounds in natural products
• Grantee: Juliane Viganó
• Support Opportunities: Scholarships in Brazil - Post-Doctoral