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Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems

Grant number: 18/25511-1
Support type:Research Projects - Thematic Grants
Duration: November 01, 2019 - October 31, 2024
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Ricardo Pinheiro de Souza Oliveira
Grantee:Ricardo Pinheiro de Souza Oliveira
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers: Attilio Converti ; Christian Hoffmann ; Danielle de Carla Dias ; Dirlei Antonio Berto ; Elias Gustavo Figueroa Villalobos ; Francisco Vitor Santos da Silva ; Iván Valdebenito Isler ; Jean Guy Joseph LeBlanc ; Jorge Gonzalo Farias Avendano ; José Manuel Domínguez González ; José Roberto Sartori ; Leonardo Tachibana ; Marcos Livio Panhoza Tse ; Maria José Tavares Ranzani de Paiva ; Patrícia Léo ; Paul Cotter ; Welington Luiz de Araújo


The use of probiotic microorganisms in the prevention and treatment of bacterial infections in animals intended for human consumption has been considered an efficient alternative to the use of antibiotics. In addition, recent studies have shown that certain substances produced by these microorganisms, such as bacteriocins, vitamins, fatty acids, exopolysaccharides, enzymes, among others, improve the immunity and development of their hosts. The most commonly used probiotic microorganisms in the food and pharmaceutical industries are those belonging to the lactic acid bacteria group (LAB), since they are considered safe by regulatory agencies in this area. However, it is known that the beneficial effects generated by probiotics are specific to each host and, frequently, the compounds of interest are synthesized in greater quantities by a given bacterial strain. The main objective of the present study is the isolation and identification of probiotic LAB present in the intestinal microbiota of poultry, swine and fish species. Strains with high capacity to produce bacteriocins will be selected. From this selection, compatibility tests will be carried out among the strains and, later, a mix of probiotics will be prepared. The strains that compose it will be individually micro-encapsulated and administered daily in the animals diet, through feed and water, in order to ascertain the probiotic effectiveness of the mixture. Synthetic bacteriocins will also be micro-encapsulated and administered to understand their individual effect on animals health. The results obtained with microcapsules will be compared with those obtained in the same biosystems in free form. In addition, we will carry out immunological studies, electron microscopy tests and analysis of the intestinal microbiota diversity of animals. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PAZ, ALICIA; COSTA-TRIGO, IVAN; OLIVEIRA, RICARDO PINHEIRO DE SOUZA; DOMINGUEZ, JOSE MANUEL. Ligninolytic Enzymes of Endospore-Forming Bacillus aryabhattai BA03. Current Microbiology, v. 77, n. 5, p. 702-709, MAY 2020. Web of Science Citations: 0.

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