Assessment of consumption of the peel of Passiflora edulis and its impact on intestinal inflammation in rats with ulcerative colitis.

• Grant number: 10/16752-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2011
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Mário Roberto Maróstica Junior
• Grantee: Cinthia Baú Betim Cazarin
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 12/24262-1 - Evaluation of the in vitro fermentation of passion fruit peel and its role in intestinal inflammation, BE.EP.DR

Abstract

Inflammatory bowel disease is a chronic relapsing disease that affects millions of people worldwide,encompassing Crohn's disease (CD) and ulcerative colitis (UC). The difference between these diseases is its location, and the UC-specific region is the colon and rectum. Current treatment for these diseases is carried out based on corticosteroids, immunomodulators or anti-TNF-alpha, known as biological therapy, which haveseveral adverse effects to the patient. The pathogenesis of this disease is related to genetic and environmental factors such as diet, smoking, drug use, stress and imbalance of the intestinal flora. It is thought that a break in the natural barrier exerted by the intestinal mucosa is the first step in triggering theinflammatory response. Once the protective barrier in the intestinal mucosa is destroyed, it turns susceptible to the effects caused by bacteria and substances present in the lumen translocation. Dietary fiber showsfunction on the modulation of microbiota and is used as substrate by microorganisms for the formation of short chain fatty acids, generated by the fermentation process. Several studies, using different sources of fiber, as well as butyrate enema, have shown positive effect in controlling the inflammatory process. Thus,this work is to evaluate the use of passion fruit peel as a source soluble fiber in the diet of rats with TNBS-induced colitis and its influence on the inflammatory process. Will be evaluated for composition of flour about the contents of dietary fiber, dietary indigestible fraction and antioxidant activity. In vivo assay will beevaluate the formation of short chain fatty acids, morphology of injured tissue, microbiota composition and activity of antioxidant enzymes, and expression of pro-inflammatory cytokines, expression of membrane receptors, MCP-1, Toll-like 2 and 4, the expression of iNOS, Cox-2 and NF-kB.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, JULIANA KELLY; BETIM CAZARIN, CINTHIA BAU; CORREA, LUIZ CLAUDIO; BATISTA, ANGELA GIOVANA; BUSCH FURLAN, CIBELE PRISCILA; TELLES BIASOTO, ALINE CAMARAO; PEREIRA, GIULIANO ELIAS; DE CAMARGO, ADRIANO COSTA; MAROSTICA JUNIOR, MARIO ROBERTO. Bioactive compounds of juices from two Brazilian grape cultivars. Journal of the Science of Food and Agriculture, v. 96, n. 6, p. 1990-1996, APR 2016. (10/16752-3, 12/17683-0)

LIMA, GLAUCIA CARIELO; CORREA VIEIRA, VIVIAN CRISTINE; BETIM CAZARIN, CINTHIA BAU; RIBEIRO, RAFAELA DA ROSA; BOGUSZ JUNIOR, STANISLAU; DE ALBUQUERQUE, CIBELE LIMA; VIDAL, RAMON OLIVEIRA; NETTO, CLAUDIA CARDOSO; YAMADA, AUREO TATSUMI; AUGUSTO, FABIO; et al. Fructooligosaccharide intake promotes epigenetic changes in the intestinal mucosa in growing and ageing rats. EUROPEAN JOURNAL OF NUTRITION, v. 57, n. 4, p. 1499-1510, JUN 2018. (10/16752-3, 10/05681-8)

DA SILVA, JULIANA KELLY; BETIM CAZARIN, CINTHIA BAU; BOGUSZ JUNIOR, STANISLAU; AUGUSTO, FABIO; MAROSTICA JUNIOR, MARIO ROBERTO. Passion fruit (Passiflora edulis) peel increases colonic production of short-chain fatty acids in Wistar rats. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 2, p. 6-pg., 2014-12-01. (10/10131-7, 10/16752-3)

DA SILVA, JULIANA KELLY; BETIM CAZARIN, CINTHIA BAU; BATISTA, ANGELA GIOVANA; MAROSTICA, JR., MARIO. Effects of passion fruit (Passiflora edulis) byproduct intake in antioxidant status of Wistar rats tissues. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 2, 2, SI, p. 1213-1219, DEC 2014. (10/16752-3, 10/10131-7)

DA SILVA, JULIANA KELLY; BETIM CAZARIN, CINTHIA BAU; BOGUSZ JUNIOR, STANISLAU; AUGUSTO, FABIO; MAROSTICA JUNIOR, MARIO ROBERTO. Passion fruit (Passiflora edulis) peel increases colonic production of short-chain fatty acids in Wistar rats. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 2, 2, SI, p. 1252-1257, DEC 2014. (10/16752-3, 10/10131-7)

DA SILVA, JULIANA KELLY; BATISTA, ANGELA GIOVANA; BETIM CAZARIN, CINTHIA BAU; DIONISIO, ANA PAULA; DE BRITO, EDY SOUSA; BIASOTO MARQUES, ALINE TELLES; MAROSTICA JUNIOR, MARIO ROBERTO. Functional tea from a Brazilian berry: Overview of the bioactives compounds. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 76, n. B, SI, p. 292-298, MAR 2017. (12/17683-0, 10/16752-3)

BETIM CAZARIN, CINTHIA BAU; DA SILVA, JULIANA KELLY; COLOMEU, TALITA CRISTINA; BATISTA, ANGELA GIOVANA; MOLINA MELETTI, LAURA MARIA; RIZZATO PASCHOAL, JONAS AUGUSTO; BOGUSZ JUNIOR, STANISLAU; DE CAMPOS BRAGA, PATRICIA APARECIDA; REYES REYES, FELIX GUILLERMO; AUGUSTO, FABIO; et al. Intake of Passiflora edulis leaf extract improves antioxidant and anti-inflammatory status in rats with 2,4,6-trinitrobenzenesulphonic acid induced colitis. Journal of Functional Foods, v. 17, p. 575-586, AUG 2015. (10/16752-3)

CAZARIN, CINTHIA B. B.; DA SILVA, JULIANA K.; COLOMEU, TALITA C.; BATISTA, ANGELA G.; VILELLA, CONCEICAO A.; FERREIRA, ANDERSON L.; BOGUSZ JUNIOR, STANISLAU; FUKUDA, KARINA; AUGUSTO, FABIO; DE MEIRELLES, LUCIANA R.; et al. Passiflora edulis peel intake and ulcerative colitis: Approaches for prevention and treatment. Experimental Biology and Medicine, v. 239, n. 5, p. 542-551, MAY 2014. (10/16752-3)

DA SILVA, JULIANA KELLY; BETIM CAZARIN, CINTHIA BAU; BATISTA, ANGELA GIOVANA; MAROSTICA, MARIO, JR.. Effects of passion fruit (Passiflora edulis) byproduct intake in antioxidant status of Wistar rats tissues. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 59, n. 2, p. 7-pg., 2014-12-01. (10/10131-7, 10/16752-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAZARIN, Cinthia Baú Betim. Evaluation of Passiflora edulis peel intake in prevention and treatment of TNBS induced ulcerative colitis. 2014. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.