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Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via feluric acid to high value chemicals

Grant number: 15/50590-4
Support type:Program for Research on Bioenergy (BIOEN) - Thematic Grants
Duration: June 01, 2017 - May 31, 2022
Field of knowledge:Biological Sciences - Genetics
Cooperation agreement: BBSRC, UKRI
Principal Investigator:Fábio Márcio Squina
Grantee:Fábio Márcio Squina
Principal investigator abroad: Tim David Howard Bugg
Institution abroad: University of Warwick, England
Home Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
Associated scholarship(s):19/26321-4 - Conversion of lignin to ferulic acid (WP2), BP.TT
18/18101-1 - Development of the CRISPR/Cas9 system in Rhodococcus jostii RHA1 for production of high-value chemicals from lignin streams, BP.DD
17/08166-6 - Genetic engineering of yeast for conversion lignocellulosic material to ferulic acid, BP.DR
+ associated scholarships 17/16089-1 - Production of lignin chains, metagenomic screening and biocatalytic conversion of lignin to ferulic acid, BP.PD
17/05901-7 - Development of E. coli-Rhodococcus CRISPR/Cas9 shuttle vector targeting ferulic acid accumulating Rhodococcus jostii RHA1 mutants, BP.MS
16/07926-4 - Novel integrative strategies through the aldoketo reductase from the termite Coptotermes gestroi for detoxification, saccharification and fermentation of lignocellulose aiming bioethanol production, BP.DR
16/09950-0 - Functional, structural characterizations and biotechnological application of lytic polysaccharide monooxygenases from the lower termite Coptotermes gestroi, BP.PD - associated scholarships

Abstract

Lignin can be obtained as a by-product of cellulosic ethanol production, and is a potential source of renewable chemicals, if methods for lignin valorization can be developed. Efficient valorisation of lignin is a major unsolved problem in the development of sustainable biorefineries. The proposal builds upon an existing BBSRC partnership award, and brings together expertise in biocatalyst discovery and lignocelullose ethanol production (CTBE) with expertise in biocatalytic lignin valorization (Warwick) and biocatalysis for high value chemicals production (Manchester, UCL) The overall aim is to generate new methods for lignin valorization via intermediate ferulic acid, which has been generated from lignin in previous Pl's works. The project will involve the following work packages: 1) optimization of lignin generation from cellulosic bioethanol; 2) conversion of lignin to ferulic acid from lignin using synthetic biology; 3) enzymatic conversion of ferulic acid into pharmaceutical L-Dopa; 4) biocatalytic generation of high value fragrance chemicals (coniferyl acetate, isoeugenol) from ferulic acid; 5) bioprocess and scale-up of chemicals production from renewable feedstocks; 6) technical and sustainability impact assessment. Technology developed in the project could be applied to major plant feedstocks used in Brazil (sugar cane) or the UK (wheat). (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Novel biotechnological route developed to obtain fine chemicals from agricultural waste 
Joint FAPESP-BBSRC Collaborative Project Meeting 
Articles published in Pesquisa para Inovação FAPESP about research grant:
Novel biotechnological route developed to obtain fine chemicals from agricultural waste 
Articles published in other media outlets (20 total):
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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TRAMONTINA, ROBSON; GALMAN, JAMES L.; PARMEGGIANI, FABIO; DERRINGTON, SASHA R.; BUGG, TIMOTHY D. H.; TURNER, NICHOLAS J.; SQUINA, FABIO M.; DIXON, NEIL. Consolidated production of coniferol and other high-value aromatic alcohols directly from lignocellulosic biomass. GREEN CHEMISTRY, v. 22, n. 1, p. 144-152, JAN 7 2020. Web of Science Citations: 0.
BRENELLI, LIVIA B.; PERSINOTI, GABRIELA F.; FRANCO CAIRO, JOAO PAULO L.; LIBERATO, MARCELO V.; GONCALVES, THIAGO AUGUSTO; OTERO, IGOR V. R.; MAINARDI, PEDRO H.; FELBY, CLAUS; SETTE, LARA D.; SQUINA, FABIO M. Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain. SCIENTIFIC REPORTS, v. 9, NOV 26 2019. Web of Science Citations: 0.
VALADARES, FERNANDA; GONCALVES, THIAGO A.; DAMASIO, ANDRE; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; SEGATO, FERNANDO; FERRAZ, ANDRE. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures. Enzyme and Microbial Technology, v. 130, NOV 2019. Web of Science Citations: 0.
PUHL, ANA C.; PRATES, ERICA T.; ROSSETO, FLAVIO R.; MANZINE, LIVIA R.; STANKOVIC, IVANA; DE ARAUJO, SIMARA S.; ALVAREZ, THABATA M.; SQUINA, FABIO M.; SKAF, MUNIR S.; POLIKARPOV, IGOR. Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern. International Journal of Biological Macromolecules, v. 136, p. 493-502, SEP 1 2019. Web of Science Citations: 0.
SODRE, VICTORIA; ARAUJO, JUSCEMACIA NASCIMENTO; GONCALVES, THIAGO AUGUSTO; VILELA, NATHALIA; KIMUS BRAZ, ANTONIO SERGIO; FRANCO, TELMA TEIXEIRA; NETO, MARIO DE OLIVEIRA; DE LIMA DAMASIO, ANDRE RICARDO; GARCIA, WANIUS; SQUINA, FABIO MARCIO. An alkaline active feruloyl-CoA synthetase from soil metagenome as a potential key enzyme for lignin valorization strategies. PLoS One, v. 14, n. 2 FEB 25 2019. Web of Science Citations: 1.
GRANJA-TRAVEZ, ROMMEL SANTIAGO; WILKINSON, RACHAEL C.; PERSINOTI, GABRIELA FELIX; SQUINA, FABIO M.; FULOP, VILMOS; BUGG, TIMOTHY D. H. Structural and functional characterisation of multi-copper oxidase CueO from lignin-degrading bacterium Ochrobactrum sp reveal its activity towards lignin model compounds and lignosulfonate. FEBS Journal, v. 285, n. 9, p. 1684-1700, MAY 2018. Web of Science Citations: 8.
PERSINOTI, GABRIELA F.; PAIXAO, DOUGLAS A. A.; BUGG, TIMOTHY D. H.; SQUINA, FABIO M. Genome Sequence of Lysinibacillus sphaericus, a Lignin-Degrading Bacterium Isolated from Municipal Solid Waste Soil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 18 MAY 2018. Web of Science Citations: 0.
MORAES, EDUARDO C.; ALVAREZ, THABATA M.; PERSINOTI, GABRIELA F.; TOMAZETTO, GEIZECLER; BRENELLI, LIVIA B.; PAIXAO, DOUGLAS A. A.; EMATSU, GABRIELA C.; ARICETTI, JULIANA A.; CALDANA, CAMILA; DIXON, NEIL; BUGG, TIMOTHY D. H.; SQUINA, FABIO M. Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization. BIOTECHNOLOGY FOR BIOFUELS, v. 11, MAR 22 2018. Web of Science Citations: 10.
LIMA, CLEILTON S.; RABELO, SARITA C.; CIESIELSKI, PETER N.; ROBERTO, INES C.; ROCHA, GEORGE J. M.; DRIEMEIER, CARLOS. Multiscale Alterations in Sugar Cane Bagasse and Straw Submitted to Alkaline Deacetylation. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 6, n. 3, p. 3796-3804, MAR 2018. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.