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Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications

Grant number: 17/02147-0
Support type:Research Projects - Thematic Grants
Duration: July 01, 2017 - June 30, 2022
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Fernando Mauro Lanças
Grantee:Fernando Mauro Lanças
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Co-Principal Investigators:Alvaro José dos Santos Neto ; Maria Eugênia Queiroz Nassur
Assoc. researchers:Gisela de Aragão Umbuzeiro ; Henrique César Santejo Silveira ; José Alexandre de Souza Crippa ; José Luiz da Costa ; Marcelo Zaiat ; Paulo Clairmont Feitosa de Lima Gomes ; Vitor Tumas
Associated grant(s):17/21985-6 - Multi-user equipment approved in grant 17/02147-0: electrospray ionization nano/micro liquid chromatography-tandem mass spectrometry system (ESI-QqQ (MS/MS)) - nano/µ-LC-ESI-MS/MS, AP.EMU
17/21984-0 - Multi-user equipment approved in grant 17/02147-0: electron ionization nano liquid chromatography-mass spectrometry system with a quadrupole analyzer (EI-Q (MS)) - nano-LC-EI-MS, AP.EMU
Associated scholarship(s):19/04386-7 - Development of an organic monolithic phase for the determination of b-amyloid peptides AB40 and AB42 in plasma samples from patients with Alzheimer's Disease by UHPLC-MS/MS in column switching mode, BP.DR
19/04057-3 - Determination of cannabinoids in vegetable oil samples by liquid-liquid extraction (LLE) and ultra-efficiency liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), BP.IC
19/02845-4 - Development of miniaturized extraction procedure based on liquid-liquid dispersive microextraction (DLLME) for analysis of 11-nor-”9-tetrahydrocannabinolcarboxylic acid in urine samples, BP.IC
+ associated scholarships 19/08168-4 - Synthesis, characterization and evaluation of new materials for extraction micro-columns and their on-line coupling with micro and nano liquid chromatography, BP.IC
18/11700-7 - Determination of pharmaceuticals in wastewater, BP.MS
18/11849-0 - The toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by oral fluid samples and characterization of their in vitro metabolism, BP.DR
18/01448-9 - Sample preparation and LC-MS for metabolomic and exposomic analyses: studies and application for multiple biological matrices, BE.PQ
18/06068-0 - Development of the column switching SALLE-UHPLC-MS/MS method to anandamide and 2-arachidonoylglycerol determination in brain samples of rats submitted to an animal model of Parkinsons Disease, BP.MS
17/26697-9 - Determination of carbidopa, 3-O-methyldopa, levodopa, benserazide and dopamine in plasma samples from parkinsonian patients by SALLE / HILIC-MS / MS method, BP.IC
17/24515-0 - Development of a microextraction by packed sorbent (MEPS) method to analysis of synthetic opioids in urine samples, BP.IC - associated scholarships

Abstract

The main motivation behind this proposal is the recognition that a well-exploited association of micro techniques covering sample preparation, chromatography and mass spectrometry exhibits very concrete potential for gains in analytical quality in association with the precepts of green chemistry. Directly, we envisage several advances from fundamental aspects to applications on real samples of relevance involving this association of analytical micro techniques. The following topics will be highlighted aiming a concrete advancement in the analytical sciences: i) the research on novel materials and strategies applied to increasing the performance of micro extractions; ii) innovation toward automation and association of analytical techniques for sample preparation, chromatography and mass spectrometry; iii) the search for novel, more efficient and robust chromatographic columns in micro and nano scales; and; iv) the evaluation, development and application of coupling of the developed micro and nano columns with mass spectrometry for higher ionization efficiency and aiming to obtain new levels of detection from the use of limited amount of complex samples. Regarding the applications of the proposed concept in chemistry and related fields, we will focus on i) biomedical applications for the analysis of endogenous biomarkers and drugs in biological fluids; ii) environmental applications in degradation studies of drugs and other emerging compounds in small scale bioreactors; iii) ecotoxicological applications for monitoring the absorption of toxicants by micro crustaceans and insect larvae, in order to correlate chemical information with other toxicological observations; (iv) food safety applications regarding fast, efficient and robust methods able to deal with difficult matrices and analytes. (AU)

Scientific publications (16)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE TOFFOLI, ANA LUCIA; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Evaluation of the tubing material and physical dimensions on the performance of extraction columns for on-line sample preparation-LC-MS/MS. Journal of Chromatography A, v. 1597, p. 18-27, JUL 19 2019. Web of Science Citations: 0.
FRANCO, MARAISSA S.; PADOVAN, RODRIGO N.; FUMES, BRUNO H.; PALMER, CHRISTOPHER P.; MCGETTRICK, JULIE R.; LANCAS, FERNANDO M. Silica modified with polymeric amphiphilic nanoparticles as first dimension for multidimensional separation techniques. Journal of Chromatography A, v. 1597, p. 149-158, JUL 19 2019. Web of Science Citations: 0.
VARGAS MEDINA, DEYBER ARLEY; RODRIGUEZ CABAL, LUIS FELIPE; TITATO, GUILHERME MIOLA; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Automated online coupling of robot-assisted single drop microextraction and liquid chromatography. Journal of Chromatography A, v. 1595, p. 66-72, JUN 21 2019. Web of Science Citations: 0.
MIRANDA, LUIS F. C.; GONCALVES, ROGERIA R.; QUEIROZ, MARIA E. C. A Dual Ligand Sol-Gel Organic-Silica Hybrid Monolithic Capillary for In-Tube SPME-MS/MS to Determine Amino Acids in Plasma Samples. Molecules, v. 24, n. 9 MAY 1 2019. Web of Science Citations: 1.
MEDINA, V, DEYBER A.; FIGUEROLA, ANDREU; RODRIGUEZ, FELIPE; SANTOS-NETO, ALVARO J.; CABELLO, CARLOS P.; PALOMINO, GEMMA T.; CERDA, VICTOR; MAYA, FERNANDO. Hyperporous carbon-coated 3D printed devices. APPLIED MATERIALS TODAY, v. 14, p. 29-34, MAR 2019. Web of Science Citations: 4.
MEJIA-CARMONA, KAREN; JORDAN-SINISTERRA, MARCELA; LANCAS, FERNANDO M. Current Trends in Fully Automated On-Line Analytical Techniques for Beverage Analysis. BEVERAGES, v. 5, n. 1 MAR 2019. Web of Science Citations: 0.
COSTA QUEIROZ, MARIA EUGENIA; DE SOUZA, ISRAEL DONIZETI; MARCHIONI, CAMILA. Current advances and applications of in-tube solid-phase microextraction. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 111, p. 261-278, FEB 2019. Web of Science Citations: 1.
QUEIROZ, MARIA EUGENIA C.; LEMOS PINTO, MONIA AP; CRUZ, JONAS CARNEIRO; USHIROHIRA, JULIANA MAYUMI; DA SILVA DIAS, ISABELLA CAROLINE; HALLAK, JAIME E. C. Schizophrenia: recent advances in LC-MS/MS methods to determine antipsychotic drugs in biological samples. BIOANALYSIS, v. 11, n. 3, p. 215-231, FEB 2019. Web of Science Citations: 1.
SOUZA, ISRAEL D.; HANTAO, LEANDRO W.; QUEIROZ, MARIA EUGENIA C. Polymeric ionic liquid open tubular capillary column for on-line in-tube SPME coupled with UHPLC-MS/MS to determine endocannabinoids in plasma samples. Analytica Chimica Acta, v. 1045, p. 108-116, JAN 3 2019. Web of Science Citations: 3.
GRECCO, CAROLINE F.; SOUZA, ISRAEL D.; ACQUARO JUNIOR, VINICIUS R.; QUEIROZ, MARIA E. C. Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry. Journal of the Brazilian Chemical Society, v. 30, n. 1, p. 48-59, JAN 2019. Web of Science Citations: 0.
SOARES MACIEL, EDVALDO VASCONCELOS; DE TOFFOLI, ANA LUCIA; LANCAS, FERNANDO MAURO. Current status and future trends on automated multidimensional separation techniques employing sorbent-based extraction columns. JOURNAL OF SEPARATION SCIENCE, v. 42, n. 1, p. 258-272, JAN 2019. Web of Science Citations: 2.
MARCHIONI, CAMILA; DE SOUZA, ISRAEL DONIZETI; ACQUARO JUNIOR, VINICIUS RICARDO; DE SOUZA CRIPPA, JOSE ALEXANDRE; TUMAS, VITOR; COSTA QUEIROZ, MARIA EUGENIA. Recent advances in LC-MS/MS methods to determine endocannabinoids in biological samples: Application in neurodegenerative diseases. Analytica Chimica Acta, v. 1044, p. 12-28, DEC 31 2018. Web of Science Citations: 6.
CARNEIRO, JUSSARA F.; SILVA, FERNANDO L.; MARTINS, ALYSSON S.; DIAS, RAFAEL M. P.; TITATO, GUILHERME M.; SANTOS-NETO, ALVARO J.; BERTAZZOLI, RODNEI; LANZA, MARCOS R. V. Simultaneous degradation of hexazinone and diuron using ZrO2-nanostructured gas diffusion electrode. CHEMICAL ENGINEERING JOURNAL, v. 351, p. 650-659, NOV 1 2018. Web of Science Citations: 0.
VARGAS MEDINA, DEYBER ARLEY; SANTOS-NETO, ALVARO JOSE; CERDA, VICTOR; MAYA, FERNANDO. Automated dispersive liquid-liquid microextraction based on the solidification of the organic phase. Talanta, v. 189, p. 241-248, NOV 1 2018. Web of Science Citations: 1.
GRECCO, CAROLINE FERNANDES; SOUZA, ISRAEL D.; COSTA QUEIROZ, MARIA EUGENIA. Recent development of chromatographic methods to determine parabens in breast milk samples: A review. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1093, p. 82-90, SEP 1 2018. Web of Science Citations: 0.

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