Application of an integrative system (2D PLExHPLC-PDA) for bioactive compound extraction and online quantification: Advantages, validation, and considerations

Author(s)

Vigano, Juliane ; Strieder, Monique Martins ; Pizani, Rodrigo S. ; Contieri, Leticia S. ; Mesquita, Leonardo M. de Souza ; Rostagno, Mauricio A.

• Document type: Journal article
• Source: Analytica Chimica Acta; v. 1272, p. 9-pg., 2023-06-15.

Abstract

Analyzing compounds such as polyphenols in solid samples frequently uses a solid-liquid extraction step. The solid-liquid extraction and analysis integration in a single equipment is not commercially available since several challenges are inherent to this hybridization. In the context of developing more sustainable analytical proced-ures, innovative techniques are demanded. Given that, this work proposes a new integrative system (2D PLE x HPLC-PDA) and presents its validation for bioactive compound extraction and online quantification, discussing the main advantages and cares that need to be taken. Two food byproducts - passion fruit bagasse and coffee husks - were chosen as solid model samples. The system was configured to perform pressurized liquid extraction (PLE) with periodical automated extract injection in the HPLC, consequently obtaining the online quantification of target compounds from the solid samples. In parallel with the online injections, extract fractions were collected and submitted to offline analysis in which the extraction yield of piceatannol and chlorogenic acid and caffeine were evaluated, respectively, for passion fruit bagasse and coffee husks. The extraction yields obtained by online and offline injections were compared and were significantly equal (p > 0.05). Thus, the 2D PLE x HPLC-PDA system represents a feasible tool to integrate solid sample preparation and chemical analysis of biocompounds in a single and online step. (AU)

• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 20/08421-9 - Application of non-volatile alternative solvents to create sustainable two-dimensional and integrated platforms for harnessing agro-industrial waste from jaboticaba and pomegranate fruits
• Grantee: Leonardo Mendes de Souza Mesquita
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/15774-5 - Development of methods of extraction, separation and two-dimensional analysis of phenolic compounds in natural products
• Grantee: Juliane Viganó
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/13496-0 - Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA)
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 21/12264-9 - A techno-economic evaluation of emerging technologies and green solvents coupling to the two-dimensional system of extraction, separation and analysis to obtain phenolic compounds from waste of the coffee and plant-based beverages industry
• Grantee: Monique Martins Strieder
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/03623-2 - Extraction and separation of phenolic compounds from green propolis by a bidimensional system
• Grantee: Letícia Sanches Contieri
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/04067-6 - Evaluation of ultrasound-assisted pressurized fluid extraction coupled with solid phase extraction for extracting phenolic compounds and terpenes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis)
• Grantee: Rodrigo Stein Pizani
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)