Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications

• Grant number: 17/02147-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: July 01, 2017
• End date: June 30, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Fernando Mauro Lanças
• Grantee: Fernando Mauro Lanças
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Pesquisadores principais: Alvaro José dos Santos Neto ; Maria Eugênia Queiroz Nassur
• Associated researchers: Gisela de Aragão Umbuzeiro ; Henrique César Santejo Silveira ; José Alexandre de Souza Crippa ; José Luiz da Costa ; Marcelo Zaiat ; Paulo Clairmont Feitosa de Lima Gomes ; Vitor Tumas
• Associated research grant(s): 17/21984-0 - Multi-user equipment approved in grant 17/02147-0: electron ionization nano liquid chromatography-mass spectrometry system with a quadrupole analyzer (EI-Q (MS)) - nano-LC-EI-MS, AP.EMU; 17/21985-6 - Multi-user equipment approved in grant 17/02147-0: electrospray ionization nano/micro liquid chromatography-tandem mass spectrometry system (ESI-QqQ (MS/MS)) - nano/µ-LC-ESI-MS/MS, AP.EMU
• Associated scholarship(s): 22/01612-9 - Planning and operation system/software development for automated microextraction equipment, BP.IC; 20/06526-8 - Development of an organic monolithic phase with anion exchanger groups and hydrophilic coating to homocysteine and homocysteic acid determination in biological samples from patients with Alzheimer's Disease by in-tube SPME-UHPLC-MS/MS, BP.DR; 20/11848-4 - Use of solid phase microextraction in tips (SPME-Tips) for analysis of cocaine and biotransformation products in oral fluid by liquid chromatography tandem mass spectrometry (LC-MS / MS), BP.IC; + associated scholarships 20/07470-6 - Development of an analytical method for the determination of ketamine and its metabolites in oral fluids samples using liquid-liquid dispersive microextration (DLLME), BP.IC; 20/07864-4 - Separation microtechniques for metabolomics and pesticide residues determination in tissues and honey of stingless native bees (Tetragonisca angustula), BP.DD; 19/22724-7 - Novel instrumental approaches for automated and miniaturized analytical methods for the detection of organic compounds by liquid chromatography coupled to mass spectrometry, BP.PD; 19/26263-4 - Development of open tubular capillary columns for micro and nano liquid chromatography coupled to sequential mass spectroscopy, BP.DD; 19/02866-1 - Development of an analytical method for the determination of methylphenidate, ethylphenidate and ritalinic acid in oral fluid samples using liquid chromatography-tandem mass spectrometry, BP.IC; 19/19485-0 - Development of nitinol fiber chemically coated with zwitterionic polymeric ionic liquids for determination of beta-amyloids in plasma samples from patients with Alzheimer's disease by direct coupling fiber-in-tube SPME and MS/MS, BP.PD; 19/04386-7 - Development of an organic monolithic phase for the determination of b-amyloid peptides AB40 and AB42 in plasma samples from patients with Alzheimer's Disease by UHPLC-MS/MS in column switching mode, BP.DR; 19/04057-3 - Determination of cannabinoids in vegetable oil samples by liquid-liquid extraction (LLE) and ultra-efficiency liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), BP.IC; 19/02845-4 - Development of miniaturized extraction procedure based on liquid-liquid dispersive microextraction (DLLME) for analysis of 11-nor-”9-tetrahydrocannabinolcarboxylic acid in urine samples, BP.IC; 19/08168-4 - Synthesis, characterization and evaluation of new materials for extraction micro-columns and their online coupling with micro and nano liquid chromatography, BP.IC; 18/11700-7 - Determination of pharmaceuticals in wastewater, BP.MS; 18/11849-0 - The toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by oral fluid samples and characterization of their in vitro metabolism, BP.DR; 18/01448-9 - Sample preparation and LC-MS for metabolomic and exposomic analyses: studies and application for multiple biological matrices, BE.PQ; 18/06068-0 - Development of the column switching SALLE-UHPLC-MS/MS method to anandamide and 2-arachidonoylglycerol determination in brain samples of rats submitted to an animal model of Parkinsons Disease, BP.MS; 17/26697-9 - Determination of carbidopa, 3-O-methyldopa, levodopa, benserazide and dopamine in plasma samples from Parkinsonian patients by SALLE / HILIC-MS / MS method, BP.IC; 17/24515-0 - Development of a microextraction by packed sorbent (MEPS) method to analysis of synthetic opioids in urine samples, BP.IC - associated scholarships

Abstract

The main motivation behind this proposal is the recognition that a well-exploited association of micro techniques covering sample preparation, chromatography and mass spectrometry exhibits very concrete potential for gains in analytical quality in association with the precepts of green chemistry. Directly, we envisage several advances from fundamental aspects to applications on real samples of relevance involving this association of analytical micro techniques. The following topics will be highlighted aiming a concrete advancement in the analytical sciences: i) the research on novel materials and strategies applied to increasing the performance of micro extractions; ii) innovation toward automation and association of analytical techniques for sample preparation, chromatography and mass spectrometry; iii) the search for novel, more efficient and robust chromatographic columns in micro and nano scales; and; iv) the evaluation, development and application of coupling of the developed micro and nano columns with mass spectrometry for higher ionization efficiency and aiming to obtain new levels of detection from the use of limited amount of complex samples. Regarding the applications of the proposed concept in chemistry and related fields, we will focus on i) biomedical applications for the analysis of endogenous biomarkers and drugs in biological fluids; ii) environmental applications in degradation studies of drugs and other emerging compounds in small scale bioreactors; iii) ecotoxicological applications for monitoring the absorption of toxicants by micro crustaceans and insect larvae, in order to correlate chemical information with other toxicological observations; (iv) food safety applications regarding fast, efficient and robust methods able to deal with difficult matrices and analytes. (AU)

Scientific publications

(93)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MEJIA-CARMONA, KAREN; MACIEL, EDVALDO VASCONCELOS SOARES; LANCAS, FERNANDO MAURO. Miniaturized liquid chromatography applied to the analysis of residues and contaminants in food: A review. ELECTROPHORESIS, v. 41, n. 20, SI, MAY 2020. (17/02147-0, 15/15462-5, 14/07347-9)

DA SILVA BURATO, JULIANA SOARES; VARGAS MEDINA, DEYBER ARLEY; DE TOFFOLI, ANA LUCIA; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Recent advances and trends in miniaturized sample preparation techniques. JOURNAL OF SEPARATION SCIENCE, v. 43, n. 1, SI, DEC 2019. (17/02147-0, 15/15462-5, 14/07347-9)

DA CUNHA, KELLY FRANCISCO; LANARO, RAFAEL; MARTINS, ALINE FRANCO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Use of injection-port derivatization for the analysis of cocaine and its metabolites in urine by gas chromatography-tandem mass spectrometry. Forensic Toxicology, AUG 2020. (17/02147-0, 18/00432-1, 18/11849-0)

SOARES MACIEL, EDVALDO VASCONCELOS; DE TOFFOLI, ANA LUCIA; LANCAS, FERNANDO MAURO. Current status and future trends on automated multidimensional separation techniques employing sorbent-based extraction columns. JOURNAL OF SEPARATION SCIENCE, v. 42, n. 1, p. 258-272, JAN 2019. (14/07347-9, 17/02147-0, 15/15462-5)

CRUZ, JONAS CARNEIRO; DE SOUZA, ISRAEL DONIZETI; GRECCO, CAROLINE FERNANDES; FIGUEIREDO, EDUARDO COSTA; QUEIROZ, MARIA EUGENIA COSTA. Recent advances in column switching high-performance liquid chromatography for bioanalysis. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 21, JUN 2021. (19/19485-0, 19/04386-7, 20/00126-8, 17/02147-0)

MACIEL, EDVALDO VASCONCELOS SOARES; MEDINA, DEYBER ARLEY VARGAS; BORSATTO, JOAO VICTOR BASSOLLI; LANCAS, FERNANDO MAURO. Towards a universal automated and miniaturized sample preparation approach. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 21, JUN 2021. (17/02147-0, 14/03765-0, 19/22724-7, 14/07347-9)

SOARES MACIEL, EDVALDO VASCONCELOS; DE TOFFOLI, ANA LUCIA; SOBIESKI, EDUARDO; DOMINGUES NAZARIO, CARLOS EDUARDO; LANCAS, FERNANDO MAURO. Miniaturized liquid chromatography focusing on analytical columns and mass spectrometry: A review. Analytica Chimica Acta, v. 1103, p. 11-31, MAR 22 2020. (17/02147-0, 15/15462-5, 14/07347-9)

MEDINA, DEYBER ARLEY VARGAS; BORSATTO, JOAO VICTOR BASSOLLI; MACIEL, EDVALDO VASCONCELOS SOARES; LANCAS, FERNANDO MAURO. Current role of modern chromatography and mass spectrometry in the analysis of mycotoxins in food. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 135, FEB 2021. (17/02147-0, 15/15462-5, 14/07347-9)

SOARES MACIEL, EDVALDO VASCONCELOS; DE TOFFOLI, ANA LUCIA; SOBIESKI NETO, EDUARDO; DOMINGUES NAZARIO, CARLOS EDUARDO; LANCAS, FERNANDO MAURO. New materials in sample preparation: Recent advances and future trends. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 119, OCT 2019. (17/02147-0, 15/15462-5, 14/07347-9)

DE CARVALHO OLIVEIRA, IGOR GUSTAVO; MARCHIONI, CAMILA; COSTA QUEIROZ, MARIA EUGENIA. Determination of anandamide in cerebrospinal fluid samples by disposable pipette extraction and ultra-high performance liquid chromatography tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1130, NOV 1 2019. (17/02147-0)

RODRIGUEZ CABAL, LUIS FELIPE; VARGAS MEDINA, DEYBER ARLEY; COSTA, JOSE LUIZ; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Determination of ring-substituted amphetamines through automated online hollow fiber liquid-phase microextraction-liquid chromatography. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 29, p. 7889-7897, NOV 2019. (17/02147-0, 18/00432-1, 16/21950-5, 10/19910-9)

COSTA QUEIROZ, MARIA EUGENIA; DE SOUZA, ISRAEL DONIZETI; DE OLIVEIRA, IGOR GUSTAVO; GRECCO, CAROLINE FERNANDES. In vivo solid phase microextraction for bioanalysis. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 153, p. 18-pg., 2022-05-04. (20/06526-8, 17/02147-0, 19/04386-7, 19/19485-0, 20/00126-8)

MEJIA-CARMONA, KAREN; LANCAS, FERNANDO M.. Modified graphene-silica as a sorbent for in-tube solid-phase microextraction coupled to liquid chromatography-tandem mass spectrometry. Determination of xanthines in coffee beverages. Journal of Chromatography A, v. 1621, JUN 21 2020. (17/02147-0, 15/15462-5, 14/07347-9)

JORDAN-SINISTERRA, MARCELA; VARGAS MEDINA, DEYBER ARLEY; LANCAS, FERNANDO MAURO. Microextraction by packed sorbent of polycyclic aromatic hydrocarbons in brewed coffee samples with a new zwitterionic ionic liquid-modified silica sorbent. Journal of Food Composition and Analysis, v. 114, p. 9-pg., 2022-08-27. (14/07347-9, 19/22724-7, 17/02147-0, 15/15462-5)

MEJIA-CARMONA, KAREN; MACIEL, EDVALDO VASCONCELOS SOARES; LANCAS, FERNANDO MAURO. Miniaturized liquid chromatography applied to the analysis of residues and contaminants in food: A review. ELECTROPHORESIS, v. 41, n. 20, p. 14-pg., 2020-05-17. (17/02147-0, 15/15462-5, 14/07347-9)

MEDINA, DEYBER ARLEY VARGAS; CARDOSO, ALESSANDRA TIMOTEO; MACIEL, EDVALDO VASCONCELOS SOARES; LANCAS, FERNANDO M.. Current materials for miniaturized sample preparation: Recent advances and future trends. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 165, p. 26-pg., 2023-06-09. (17/02147-0, 19/22724-7, 17/21984-0, 17/21985-6)

BORSATTO, JOAO VICTOR BASOLLI; MACIEL, EDVALDO VASCONCELOS SOARES; CIFUENTES, ALEJANDRO; LANCAS, FERNANDO MAURO. Applicability and Limitations of a Capillary-LC Column-Switching System Using Hybrid Graphene-Based Stationary Phases. Molecules, v. 28, n. 13, p. 14-pg., 2023-07-01. (17/02147-0, 19/22724-7)

SANTOS, NATALIA G. P.; MEDINA, DEYBER A. V.; LANCAS, FERNANDO M.. Development of Wall-Coated Open Tubular Columns and Their Application to Nano Liquid Chromatography Coupled to Tandem Mass Spectrometry. Molecules, v. 28, n. 13, p. 15-pg., 2023-07-01. (15/15462-5, 19/22724-7, 17/02147-0, 14/07347-9, 19/26263-4)

DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Green analytical toxicology method for determination of synthetic cathinones in oral fluid samples by microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry. Forensic Toxicology, v. N/A, p. 13-pg., 2023-07-28. (17/02147-0, 20/10809-5, 18/11849-0, 18/00432-1)

PINTO, MANIA A. L.; DE SOUZA, ISRAEL D.; MIRANDA, LUIS FELIPPE C.; QUEIROZ, MARIA EUGENIA C.. A New Molecularly Imprinted Polymer for In-Tube SPME/UHPLC-MS/MS of Anandamide in Plasma Samples. Journal of the Brazilian Chemical Society, v. N/A, p. 9-pg., 2023-05-16. (13/03808-9, 15/07619-1, 17/02147-0, 20/00126-8, 19/19485-0)

SARTORE, DOUGLAS M.; MEDINA, DEYBER VARGAS A.; BOCELLI, MARCIO D.; JORDAN-SINISTERRA, MARCELA; SANTOS-NETO, ALVARO J.; LANCAS, FERNANDO M.. Modern automated microextraction procedures for bioanalytical, environmental, and food analyses. JOURNAL OF SEPARATION SCIENCE, v. 46, n. 15, p. 30-pg., 2023-05-26. (17/02147-0, 19/22724-7, 15/15462-5, 14/07347-9)

BOCELLI, MARCIO DAVID; MEDINA, DEYBER ARLEY VARGAS; LANCAS, FERNANDO MAURO; DOS SANTOS-NETO, ALVARO JOSE. Automated microextraction by packed sorbent of endocrine disruptors in wastewater using a high-throughput robotic platform followed by liquid chromatography-tandem mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. N/A, p. 12-pg., 2023-08-03. (17/02147-0, 19/22724-7, 15/15462-5, 14/07347-9)

MEDINA, DEYBER ARLEY VARGAS; MACIEL, EDVALDO VASCONCELOS SOARES; LANCAS, FERNANDO MAURO. Modern automated sample preparation for the determination of organic compounds: A review on robotic and on-flow systems. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 166, p. 21-pg., 2023-09-01. (17/02147-0, 19/22724-7, 17/21984-0, 17/21985-6)

MEDINA, DEYBER ARLEY VARGAS; CARDOSO, ALESSANDRA TIMOTEO; BORSATTO, JOAO VICTOR BASOLLI; LANCAS, FERNANDO MAURO. Open tubular liquid chromatography: Recent advances and future trends. JOURNAL OF SEPARATION SCIENCE, v. N/A, p. 22-pg., 2023-08-15. (17/02147-0, 19/22724-7)

DE SOUZA, ISRAEL DONIZETI; QUEIROZ, MARIA EUGENIA COSTA. Advances in sample preparation and HPLC-MS/MS methods for determining amyloid-beta peptide in biological samples: a review. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 415, n. 18, p. 19-pg., 2023-03-06. (17/02147-0, 19/19485-0, 20/00126-8)

GRECCO, CAROLINE FERNANDES; DE SOUZA, ISRAEL DONIZETI; QUEIROZ, MARIA EUGENIA COSTA. Novel materials as capillary coatings for in-tube solid-phase microextraction for bioanalysis. JOURNAL OF SEPARATION SCIENCE, v. 44, n. 8, p. 1662-1693, APR 2021. (17/02147-0, 20/00126-8, 19/04386-7, 19/19485-0)

MACIEL, EDVALDO VASCONCELOS SOARES; MEJIA-CARMONA, KAREN; JORDAN-SINISTERRA, MARCELA; DA SILVA, LUIS FELIPE; VARGAS MEDINA, DEYBER ARLEY; LANCAS, FERNANDO MAURO. The Current Role of Graphene-Based Nanomaterials in the Sample Preparation Arena. FRONTIERS IN CHEMISTRY, v. 8, AUG 11 2020. (17/02147-0, 15/15462-5, 14/07347-9)

GRECCO, CAROLINE FERNANDES; MIRANDA, LUIS FELIPPE CABRAL; QUEIROZ, MARIA EUGENIA COSTA. Aminopropyl hybrid silica monolithic capillary containing mesoporous SBA-15 particles for in-tube SPME-HILIC-MS/MS to determine levodopa, carbidopa, benserazide, dopamine, and 3-O-methyldopa in plasma samples. Microchemical Journal, v. 157, SEP 2020. (17/02147-0, 17/03726-3)

SARTORE, DOUGLAS MORISUE; COSTA, JOSE LUIZ; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Packed in-tube SPME-LC-MS/MS for fast and straightforward analysis of cannabinoids and metabolites in human urine. ELECTROPHORESIS, v. 43, n. 15, p. 12-pg., 2022-04-29. (17/02147-0)

BOCELLI, MARCIO DAVID; VARGAS MEDINA, DEYBER ARLEY; GARCIA RODRIGUEZ, JULIE PAULIN; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Determination of parabens in wastewater samples via robot-assisted dynamic single-drop microextraction and liquid chromatography-tandem mass spectrometry. ELECTROPHORESIS, v. 43, n. 15, p. 10-pg., 2022-05-26. (17/02147-0, 19/22724-7, 14/07347-9, 15/15462-5)

MEDINA, DEYBER ARLEY VARGAS; BURATO, JULIANA SOARES DA SILVA; BORSATTO, JOAO VICTOR BASOLLI; LANCAS, FERNANDO MAURO. Porous layer open tubular nano liquid chromatography directly coupled to electron ionization mass spectrometry. Journal of Chromatography A, v. 1674, p. 9-pg., 2022-05-16. (17/02147-0, 19/22724-7, 14/07347-9, 15/15462-5)

DA SILVA, LUIS FELIPE; LANCAS, FERNANDO MAURO. beta-Cyclodextrin coupled to graphene oxide supported on aminopropyl silica as a sorbent material for determination of isoflavones. JOURNAL OF SEPARATION SCIENCE, v. 43, n. 23, OCT 2020. (17/02147-0)

SOUZA, ISRAEL D.; HANTAO, LEANDRO W.; QUEIROZ, MARIA EUGENIA C.. Polymeric ionic liquid open tubular capillary column for on-line in-tube SPME coupled with UHPLC-MS/MS to determine endocannabinoids in plasma samples. Analytica Chimica Acta, v. 1045, p. 108-116, JAN 3 2019. (17/02147-0, 16/01082-9, 15/07619-1)

SOUZA, ISRAEL D.; OLIVEIRA, IGOR G. C.; QUEIROZ, MARIA EUGENIA C.. Innovative extraction materials for fiber-in-tube solid phase microextraction: A review. Analytica Chimica Acta, v. 1165, JUN 22 2021. (20/00126-8, 18/06068-0, 17/02147-0, 19/19485-0)

SOUZA, ISRAEL D.; ANDERSON, JARED L.; QUEIROZ, MARIA EUGENIA C.. Crosslinked zwitterionic polymeric ionic liquid-functionalized nitinol wires for fiber-in-tube solid-phase microextraction and UHPLC-MS/MS as an amyloid beta peptide binding protein assay in biological fluids. Analytica Chimica Acta, v. 1193, FEB 8 2022. (17/02147-0, 19/19485-0, 20/00126-8)

VARGAS MEDINA, DEYBER ARLEY; RODRIGUEZ CABAL, LUIS FELIPE; TITATO, GUILHERME MIOLA; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Automated online coupling of robot-assisted single drop microextraction and liquid chromatography. Journal of Chromatography A, v. 1595, p. 66-72, JUN 21 2019. (17/02147-0, 10/19910-9, 16/21950-5)

MEDINA, V, DEYBER A.; FIGUEROLA, ANDREU; RODRIGUEZ, FELIPE; SANTOS-NETO, ALVARO J.; CABELLO, CARLOS P.; PALOMINO, GEMMA T.; CERDA, VICTOR; MAYA, FERNANDO. Hyperporous carbon-coated 3D printed devices. APPLIED MATERIALS TODAY, v. 14, p. 29-34, MAR 2019. (17/02147-0, 10/19910-9)

BELOTI, LUIZ G. M.; MIRANDA, LUIS F. C.; QUEIROZ, MARIA EUGENIA C.. Butyl Methacrylate-Co-Ethylene Glycol Dimethacrylate Monolith for Online in-Tube SPME-UHPLC-MS/MS to Determine Chlopromazine, Clozapine, Quetiapine, Olanzapine, and Their Metabolites in Plasma Samples. Molecules, v. 24, n. 2, JAN 2 2019. (17/02147-0)

MARCHIONI, CAMILA; VIEIRA, TATIANA MANZINI; MILLER CROTTI, ANTONIO EDUARDO; CRIPPA, JOSE ALEXANDRE; COSTA QUEIROZ, MARIA EUGENIA. In-tube solid-phase microextraction with a dummy molecularly imprinted monolithic capillary coupled to ultra-performance liquid chromatography-tandem mass spectrometry to determine cannabinoids in plasma samples. Analytica Chimica Acta, v. 1099, p. 145-154, FEB 22 2020. (17/02147-0, 16/13639-8)

MIRANDA, LUIS F. C.; GONCALVES, ROGERIA R.; QUEIROZ, MARIA E. C.. A Dual Ligand Sol-Gel Organic-Silica Hybrid Monolithic Capillary for In-Tube SPME-MS/MS to Determine Amino Acids in Plasma Samples. Molecules, v. 24, n. 9, MAY 1 2019. (17/02147-0)

VARGAS MEDINA, DEYBER ARLEY; PEREIRA DOS SANTOS, NATALIA GABRIELLY; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Current prospects on nano liquid chromatography coupled to electron ionization mass spectrometry (nanoLC-EI-MS). Journal of Liquid Chromatography & Related Technologies, v. 44, n. 17-20, p. 10-pg., 2022-08-17. (19/26263-4, 19/22724-7, 17/02147-0, 14/03765-0, 15/15462-5, 14/07347-9)

SOUZA, ISRAEL D.; ANDERSON, JARED L.; TUMAS, VITOR; QUEIROZ, MARIA EUGENIA C.. Direct coupling of fiber-in-tube solid-phase microextraction with tandem mass spectrometry to determine amyloid beta peptides as biomarkers for Alzheimer's disease in cerebrospinal fluid samples. Talanta, v. 254, p. 8-pg., 2023-03-01. (19/19485-0, 20/00126-8, 17/02147-0)

CRUZ, JONAS CARNEIRO; DE SOUZA, ISRAEL DONIZETI; LANCAS, FERNANDO MAURO; COSTA QUEIROZ, MARIA EUGENIA. Current advances and applications of online sample preparation techniques for miniaturized liquid chromatography systems. Journal of Chromatography A, v. 1668, p. 16-pg., 2022-04-12. (19/19485-0, 17/02147-0, 20/00126-8)

OLIVEIRA, THAIS CAMAROTTO; LANCAS, FERNANDO MAURO. Determination of selected herbicides in sugarcane-derived foods by graphene-oxide based disposable pipette extraction followed by liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, v. 1687, p. 11-pg., 2023-01-04. (17/02147-0, 19/22724-7, 14/07347-9, 15/15462-5)

BORSATTO, JOAO V. B.; MACIEL, EDVALDO V. S.; LANCAS, FERNANDO M.. Investigation of the applicability of silica-graphene hybrid materials as stationary phases for capillary liquid chromatography. Journal of Chromatography A, v. 1685, p. 8-pg., 2022-11-04. (17/02147-0, 19/22724-7, 14/07347-9, 15/15462-5)

CRUZ, JONAS CARNEIRO; DE FARIA, HENRIQUE DIPE; FIGUEIREDO, EDUARDO COSTA; QUEIROZ, MARIA EUGENIA COSTA. Restricted access carbon nanotube for microextraction by packed sorbent to determine antipsychotics in plasma samples by high-performance liquid chromatography-tandem mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 412, n. 11, SI, FEB 2020. (17/02147-0)

MACIEL, EDVALDO V. S.; BORSATTO, JOAO V. B.; MEJIA-CARMONA, KAREN; LANCAS, FERNANDO M.. Application of an in-house packed octadecylsilica-functionalized graphene oxide column for capillary liquid chromatography analysis of hormones in urine samples. Analytica Chimica Acta, v. 1239, p. 10-pg., 2022-12-20. (17/02147-0)

DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS. JOURNAL OF ANALYTICAL TOXICOLOGY, v. 44, n. 7, p. 697-707, SEP 2020. (17/02147-0, 18/11849-0, 18/00432-1)

RODRIGUEZ CABAL, LUIS FELIPE; VARGAS MEDINA, DEYBER ARLEY; LIMA, ADRIEL MARTINS; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Robotic-assisted dynamic large drop microextraction. Journal of Chromatography A, v. 1608, DEC 20 2019. (17/02147-0, 16/21950-5, 10/19910-9)

ACQUARO JUNIOR, VINICIUS R.; GOMEZ-RIOS, GERMAN AUGUSTO; TASCON, MARCOS; COSTA QUEIROZ, MARIA EUGENIA; PAWLISZYN, JANUSZ. Analysis of endocannabinoids in plasma samples by biocompatible solid-phase microextraction devices coupled to mass spectrometry. Analytica Chimica Acta, v. 1091, p. 135-145, DEC 24 2019. (17/02147-0, 16/16180-6)

DA CUNHA, KELLY FRANCISCO; RODRIGUES, LEONARDO COSTALONGA; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Miniaturized extraction method for analysis of synthetic opioids in urine by microextraction with packed sorbent and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, v. 1624, AUG 2 2020. (17/02147-0, 18/11849-0, 17/24515-0, 18/00432-1)

VASCONCELOS SOARES MACIEL, EDVALDO; MEJIA-CARMONA, KAREN; LANCAS, FERNANDO MAURO. Evaluation of Two Fully Automated Setups for Mycotoxin Analysis Based on Online Extraction-Liquid Chromatography-Tandem Mass Spectrometry. Molecules, v. 25, n. 12, JUN 2020. (17/02147-0, 15/15462-5, 14/07347-9)

DA CUNHA, KELLY FRANCISCO; OLIVEIRA, KARINA DINIZ; CARDOSO, MARILIA SANTORO; FURIOZO ARANTES, ANA CAROLINA; PIRAS COSER, PEDRO HENRIQUE; LIMA, LUCAS DE NORONHA; SAMPAIO MALUF, ANA CRISTHINA; DE CASTRO COMIS, MARIA ANGELICA; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. Prevalence of new psychoactive substances (NPS) in Brazil based on oral fluid analysis of samples collected at electronic music festivals and parties. DRUG AND ALCOHOL DEPENDENCE, v. 227, OCT 1 2021. (17/02147-0, 18/11849-0, 18/00432-1)

MAGALHAES KAHL, JULIA MARTINELLI; DA CUNHA, KELLY FRANCISCO; RODRIGUES, LEONARDO COSTALONGA; CHINAGLIA, KAUE DE OLIVEIRA; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Quantification of amphetamine and derivatives in oral fluid by dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, v. 196, MAR 20 2021. (17/02147-0, 18/00432-1)

VARGAS MEDINA, DEYBER ARLEY; SOARES MACIEL, EDVALDO VASCONCELOS; DE TOFFOLI, ANA LUCIA; LANCAS, FERNANDO MAURO. Miniaturization of liquid chromatography coupled to mass spectrometry. 2. Achievements on modern instrumentation for miniaturized liquid chromatography coupled to mass spectrometry. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 128, JUL 2020. (17/02147-0, 15/15462-5, 14/07347-9, 19/22724-7)

VARGAS MEDINA, DEYBER ARLEY; PEREIRA DOS SANTOS, NATALIA GABRIELLY; DA SILVA BURATO, JULIANA SOARES; BASOLLI BORSATTO, JOAO VICTOR; LANCAS, FERNANDO MAURO. An overview of open tubular liquid chromatography with a focus on the coupling with mass spectrometry for the analysis of small molecules. Journal of Chromatography A, v. 1641, MAR 29 2021. (19/26263-4, 19/22724-7, 14/07347-9, 17/02147-0, 14/03765-0, 15/15462-5)

DE CAMPOS, VICTORIA M.; ANDRADE, MARIANE A.; MACIEL, EDVALDO V. S.; DE TOFFOLI, ANA LUCIA; LANCAS, FERNANDO M.. Environmentally friendly analysis of sulphonamides in Brazilian honey through automated and miniaturised sample preparation coupled with LC-MS/MS. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 39, n. 5, p. 13-pg., 2022-03-25. (17/02147-0)

MARCHIONI, CAMILA; DE SOUZA, ISRAEL DONIZETI; ACQUARO JUNIOR, VINICIUS RICARDO; DE SOUZA CRIPPA, JOSE ALEXANDRE; TUMAS, VITOR; COSTA QUEIROZ, MARIA EUGENIA. Recent advances in LC-MS/MS methods to determine endocannabinoids in biological samples: Application in neurodegenerative diseases. Analytica Chimica Acta, v. 1044, p. 12-28, DEC 31 2018. (17/02147-0, 15/07619-1, 16/13639-8)

GRECCO, CAROLINE F.; SOUZA, ISRAEL D.; ACQUARO JUNIOR, VINICIUS R.; QUEIROZ, MARIA E. C.. Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry. Journal of the Brazilian Chemical Society, v. 30, n. 1, p. 48-59, JAN 2019. (17/02147-0, 15/04060-3, 15/07619-1)

VARGAS MEDINA, DEYBER ARLEY; SANTOS-NETO, ALVARO JOSE; CERDA, VICTOR; MAYA, FERNANDO. Automated dispersive liquid-liquid microextraction based on the solidification of the organic phase. Talanta, v. 189, p. 241-248, NOV 1 2018. (17/02147-0, 10/19910-9, 16/21950-5)

CARNEIRO, JUSSARA F.; SILVA, FERNANDO L.; MARTINS, ALYSSON S.; DIAS, RAFAEL M. P.; TITATO, GUILHERME M.; SANTOS-NETO, ALVARO J.; BERTAZZOLI, RODNEI; LANZA, MARCOS R. V.. Simultaneous degradation of hexazinone and diuron using ZrO2-nanostructured gas diffusion electrode. CHEMICAL ENGINEERING JOURNAL, v. 351, p. 650-659, NOV 1 2018. (17/10118-0, 14/50945-4, 17/02147-0, 16/01937-4, 11/14314-1)

DA SILVA BURATO, JULIANA SOARES; VARGAS MEDINA, DEYBER ARLEY; DE TOFFOLI, ANA LUCIA; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Recent advances and trends in miniaturized sample preparation techniques. JOURNAL OF SEPARATION SCIENCE, v. 43, n. 1, SI, p. 202-225, JAN 2020. (17/02147-0, 15/15462-5, 14/07347-9)

MEJIA-CARMONA, KAREN; DA SILVA BURATO, JULIANA SOARES; BASOLLI BORSATTO, JOAO VICTOR; DE TOFFOLI, ANA LUCIA; LACAS, FERNANDO MAURO. Miniaturization of liquid chromatography coupled to mass spectrometry 1. Current trends on miniaturized LC columns. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 122, JAN 2020. (17/02147-0, 15/15462-5, 14/07347-9)

SARTORE, DOUGLAS MORISUE; VARGAS MEDINA, DEYBER ARLEY; COSTA, JOSE LUIZ; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Automated microextraction by packed sorbent of cannabinoids from human urine using a lab-made device packed with molecularly imprinted polymer. Talanta, v. 219, NOV 1 2020. (17/02147-0, 19/22724-7, 10/19910-9)

AKAMINE, LIDIA AKEMI; MEDINA, DEYBER ARLEY VARGAS; LANCAS, FERNANDO MAURO. Magnetic solid-phase extraction of gingerols in ginger containing products. Talanta, v. 222, JAN 15 2021. (17/02147-0, 10/19910-9, 19/22724-7)

DA SILVA, LUIS FELIPE; VARGAS MEDINA, DEYBER ARLEY; LANCAS, FERNANDO MAURO. Automated needle-sleeve based online hyphenation of solid-phase microextraction and liquid chromatography. Talanta, v. 221, JAN 1 2021. (14/07347-9, 14/03765-0, 15/15462-5, 17/02147-0, 19/22724-7)

DE TOFFOLI, ANA LUCIA; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Evaluation of the tubing material and physical dimensions on the performance of extraction columns for on-line sample preparation-LC-MS/MS. Journal of Chromatography A, v. 1597, p. 18-27, JUL 19 2019. (17/02147-0, 15/15462-5, 14/07347-9)

FRANCO, MARAISSA S.; PADOVAN, RODRIGO N.; FUMES, BRUNO H.; PALMER, CHRISTOPHER P.; MCGETTRICK, JULIE R.; LANCAS, FERNANDO M.. Silica modified with polymeric amphiphilic nanoparticles as first dimension for multidimensional separation techniques. Journal of Chromatography A, v. 1597, p. 149-158, JUL 19 2019. (17/02147-0, 15/15462-5, 14/07347-9, 15/03998-8)

MEJIA-CARMONA, KAREN; JORDAN-SINISTERRA, MARCELA; LANCAS, FERNANDO M.. Current Trends in Fully Automated On-Line Analytical Techniques for Beverage Analysis. BEVERAGES, v. 5, n. 1, MAR 2019. (17/02147-0, 15/15462-5, 14/07347-9)

GRECCO, CAROLINE FERNANDES; SOUZA, ISRAEL D.; COSTA QUEIROZ, MARIA EUGENIA. Recent development of chromatographic methods to determine parabens in breast milk samples: A review. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1093, p. 82-90, SEP 1 2018. (17/03726-3, 17/02147-0)

SOARES MACIEL, EDVALDO VASCONCELOS; DE TOFFOLI, ANA LUCIA; ALVES, JUSSARA DA SILVA; LANCAS, FERNANDO MAURO. Multidimensional Liquid Chromatography Employing a Graphene Oxide Capillary Column as the First Dimension: Determination of Antidepressant and Antiepileptic Drugs in Urine. Molecules, v. 25, n. 5, MAR 1 2020. (17/02147-0, 15/15462-5, 14/07347-9)

BORSATTO, JOAO V. B.; MACIEL, EDVALDO V. S.; CIFUENTES, ALEJANDRO; LANCAS, FERNANDO M.. Online Extraction Followed by LC-MS/MS Analysis of Lipids in Natural Samples: A Proof-of-Concept Profiling Lecithin in Seeds. FOODS, v. 12, n. 2, p. 12-pg., 2023-01-01. (17/02147-0, 19/22724-7)

COSTA QUEIROZ, MARIA EUGENIA; DE SOUZA, ISRAEL DONIZETI; MARCHIONI, CAMILA. Current advances and applications of in-tube solid-phase microextraction. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 111, p. 261-278, FEB 2019. (17/02147-0)

QUEIROZ, MARIA EUGENIA C.; LEMOS PINTO, MONIA AP; CRUZ, JONAS CARNEIRO; USHIROHIRA, JULIANA MAYUMI; DA SILVA DIAS, ISABELLA CAROLINE; HALLAK, JAIME E. C.. Schizophrenia: recent advances in LC-MS/MS methods to determine antipsychotic drugs in biological samples. BIOANALYSIS, v. 11, n. 3, p. 215-231, FEB 2019. (17/02147-0)

VARGAS MEDINA, DEYBER ARLEY; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Miniaturization of liquid chromatography coupled to mass spectrometry. 3. Achievements on chip-based LC-MS devices. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 131, OCT 2020. (19/22724-7, 14/03765-0, 15/15462-5, 17/02147-0, 14/07347-9)

CARVALHO OLIVEIRA, IGOR GUSTAVO; COSTA QUEIROZ, MARIA EUGENIA. A micro salting-out assisted liquid-liquid extraction combined with ultra-high performance liquid chromatography tandem mass spectrometry to determine anandamide and 2-arachidonoylglycerol in rat brain samples. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1158, NOV 20 2020. (14/50891-1, 17/02147-0)

SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. cartridge-based device for automated analyses of solid matrices by online sample prep-capillary LC-MS/M. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 414, n. 8, SI, p. 2725-2737, MAR 2022. (17/02147-0)

CRUZ, JONAS CARNEIRO; CABRAL MIRANDA, LUIS FELIPPE; COSTA QUEIROZ, MARIA EUGENIA. Pipette tip micro-solid phase extraction (octyl-functionalized hybrid silica monolith) and ultra-high-performance liquid chromatography-tandem mass spectrometry to determine cannabidiol and tetrahydrocannabinol in plasma samples. JOURNAL OF SEPARATION SCIENCE, v. 44, n. 8, JAN 2021. (17/02147-0)

SOARES MACIEL, EDVALDO VASCONCELOS; PEREIRA DOS SANTOS, NATALIA GABRIELLY; VARGAS MEDINA, DEYBER ARLEY; LANCAS, FERNANDO MAURO. Electron ionization mass spectrometry: Quo vadis?. ELECTROPHORESIS, v. 43, n. 15, p. 14-pg., 2022-05-20. (19/26263-4, 19/22724-7, 17/02147-0, 14/03765-0, 15/15462-5, 14/07347-9)

DOS SANTOS, NATALIA GABRIELLY; MEDINA, DEYBER ARLEY VARGAS; LANCAS, FERNANDO MAURO. Microextraction by packed sorbent of N-nitrosamines from Losartan tablets using a high-throughput robot platform followed by liquid chromatography-tandem mass spectrometry. JOURNAL OF SEPARATION SCIENCE, v. 46, n. 17, p. 13-pg., 2023-07-03. (15/15462-5, 19/22724-7, 17/02147-0, 14/07347-9, 19/26263-4)

DOS SANTOS, NATALIA GABRIELLY PEREIRA; MACIEL, EDVALDO VASCONCELOS SOARES; MEDINA, DEYBER ARLEY VARGAS; LANCAS, FERNANDO MAURO. NanoLC-EI-MS: Perspectives in Biochemical Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 14, p. 14-pg., 2023-07-01. (19/22724-7, 17/21984-0, 17/02147-0, 17/21985-6, 19/26263-4)

GRECCO, CAROLINE FERNANDES; TUMAS, VITOR; HALLAK, JAIME EDUARDO CECILIO; QUEIROZ, MARIA EUGENIA COSTA. In-tube solid-phase microextraction (sulfopropyl methacrylate-co-ethylene glycol dimethacrylate monolithic capillary) coupled to LC-MS/MS to determine beta amyloid peptides in Alzheimer's cerebrospinal fluid samples. SEPARATION SCIENCE PLUS, v. N/A, p. 12-pg., 2023-08-07. (17/02147-0, 20/00126-8, 19/04386-7)

ANDRADE, FELIPE N.; SANTOS-NETO, ALVARO JOSE; MEDINA, DEYBER ARLEY V.; LANCAS, FERNANDO M.. A molecularly imprinted polymer for microextraction by packed sorbent of sulfonylureas herbicides from corn samples. Journal of Food Composition and Analysis, v. 121, p. 7-pg., 2023-05-12. (15/15462-5, 11/09898-4, 19/22724-7, 17/02147-0, 14/07347-9, 13/17425-4)

GRECCO, CAROLINE FERNANDES; DE SOUZA, ISRAEL DONIZETI; OLIVEIRA, IGOR GUSTAVO CARVALHO; QUEIROZ, MARIA EUGENIA COSTA. In-Tube Solid-Phase Microextraction Directly Coupled to Mass Spectrometric Systems: A Review. SEPARATIONS, v. 9, n. 12, p. 19-pg., 2022-12-01. (17/02147-0, 19/19485-0, 20/00126-8, 20/06526-8, 19/04386-7)

OLIVEIRA, THAIS CAMAROTTO; LANCAS, FERNANDO MAURO. Determination of selected herbicides employed in sugarcane crops by disposable pipette tip (DPX) extraction followed by LC-MS/MS and GC-MS/MS. Journal of Liquid Chromatography & Related Technologies, v. 44, n. 17-20, p. 8-pg., 2022-08-22. (17/02147-0, 14/07347-9, 19/22724-7, 15/15462-5)

DA SILVA BURATO, JULIANA SOARES; BASOLLI BORSATTO, JOAO VICTOR; LANCAS, FERNANDO MAURO. Practical aspects of preparation, optimization, and evaluation of poly (styrene-divinylbenzene)porous-layer open tubular (PLOT) columns for capillary liquid chromatography. Talanta, v. 253, p. 7-pg., 2023-02-01. (17/02147-0, 14/07347-9, 19/22724-7, 15/15462-5)

BORSATTO, JOAO V. B.; LANCAS, FERNANDO M. M.. Recent Trends in Graphene-Based Sorbents for LC Analysis of Food and Environmental Water Samples. Molecules, v. 28, n. 13, p. 21-pg., 2023-07-01. (17/02147-0, 19/22724-7)

DA CUNHA, KELLY FRANCISCO; LANARO, RAFAEL; MARTINS, ALINE FRANCO; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. Use of injection-port derivatization for the analysis of cocaine and its metabolites in urine by gas chromatography-tandem mass spectrometry. Forensic Toxicology, v. 39, n. 1, p. 8-pg., 2020-08-01. (17/02147-0, 18/11849-0, 18/00432-1)

CAROLINE F. GRECCO; EDUARDO JOSÉ CREVELIN; VITOR TUMAS; JAIME EDUARDO C. HALLAK; MARIA EUGÊNIA C. QUEIROZ. Disposable Pipette Extraction Followed by Direct MS/MS Analysis of Beta Amyloid Peptides (Aß38, Aß40, and Aβ42) in Cerebrospinal Fluid Samples. Journal of the Brazilian Chemical Society, v. 36, n. 1, 2024-06-17. (17/02147-0, 20/00126-8, 19/04386-7)

MEDINA, DEYBER ARLEY VARGAS; MACIEL, EDVALDO VASCONCELOS SOARES; DOS SANTOS, NATALIA GABRIELLY PEREIRA; LANCAS, FERNANDO MAURO. The overshadowed role of electron ionization-mass spectrometry in analytical biotechnology. CURRENT OPINION IN BIOTECHNOLOGY, v. 82, p. 9-pg., 2023-06-30. (17/02147-0, 19/22724-7)

VARGAS MEDINA, DEYBER ARLEY; RODRIGUEZ CABAL, LUIS FELIPE; LANCAS, FERNANDO MAURO; SANTOS-NETO, ALVARO JOSE. Sample treatment platform for automated integration of microextraction techniques and liquid chromatography analysis. HARDWAREX, v. 5, p. 16-pg., 2019-04-01. (17/02147-0, 10/19910-9, 14/03765-0)

DA SILVA BURATO, JULIANA SOARES; VARGAS MEDINA, DEYBER ARLEY; DE TOFFOLI, ANA LUCIA; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Recent advances and trends in miniaturized sample preparation techniques. JOURNAL OF SEPARATION SCIENCE, v. 43, n. 1, p. 24-pg., 2019-12-23. (17/02147-0, 15/15462-5, 14/07347-9)

CRUZ, JONAS CARNEIRO; DE FARIA, HENRIQUE DIPE; FIGUEIREDO, EDUARDO COSTA; QUEIROZ, MARIA EUGENIA COSTA. Restricted access carbon nanotube for microextraction by packed sorbent to determine antipsychotics in plasma samples by high-performance liquid chromatography-tandem mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 412, n. 11, p. 11-pg., 2020-02-05. (17/02147-0)